目的 通過(guò)整合藥理學(xué)平臺(tái)尋找金鈴子散治療消化性潰瘍的質(zhì)量標(biāo)志物并探討其作用機(jī)制。方法 以整合藥理學(xué)平臺(tái)為基礎(chǔ)建立金鈴子散"藥材-成分""疾病-靶標(biāo)-成分"數(shù)據(jù)庫(kù)，篩選關(guān)鍵靶標(biāo)并進(jìn)行通路富集分析，以Score ≥ 0.8為相似度，采用AI技術(shù)對(duì)數(shù)據(jù)庫(kù)進(jìn)行分析和相互關(guān)聯(lián)，進(jìn)行金鈴子散治療消化性潰瘍的"中藥-成分-靶標(biāo)-通路"多維網(wǎng)絡(luò)分析，篩選出金鈴子散抗?jié)兊馁|(zhì)量標(biāo)志物，并對(duì)其分子機(jī)制進(jìn)行預(yù)測(cè)。結(jié)果 通過(guò)整合藥理學(xué)平臺(tái)中藥成分?jǐn)?shù)據(jù)庫(kù)搜索，川楝子共收集7個(gè)成分，主要為三萜類成分，共預(yù)測(cè)到2個(gè)潛在的疾病靶標(biāo)；延胡索共收集31個(gè)化學(xué)成分，主要為生物堿類成分，共預(yù)測(cè)到9個(gè)潛在疾病靶標(biāo)。經(jīng)篩選與通路富集分析，篩選出與治療消化性潰瘍相關(guān)的關(guān)鍵靶標(biāo)7個(gè)RAC1、RAC2、NCF1、NCF2、NCF4、CYBA、ATP1A1，基因功能主要有血管內(nèi)皮生長(zhǎng)因子（VEGF）受體信號(hào)通路、谷氨酸受體活性及相關(guān)通道、磷脂酰肌醇、血小板活化及其生長(zhǎng)因子、Ras鳥苷核苷酸交換因子活性等。對(duì)數(shù)據(jù)庫(kù)進(jìn)行分析互聯(lián)，最后篩選出海罌粟堿為金鈴子散抗?jié)兊馁|(zhì)量標(biāo)志物，金鈴子散可能是通過(guò)神經(jīng)系統(tǒng)、免疫系統(tǒng)、細(xì)胞通信、RAS通路、RAP1通路及黏合連接通路等達(dá)到治療潰瘍效果。結(jié)論 金鈴子散應(yīng)以延胡索和海罌粟堿做為進(jìn)一步研究抗消化性潰瘍的主要目標(biāo)。

Objective To find the quality marker and explore the mechanism of peptic ulcer by integrating pharmacology platform. Methods Based on integrated pharmacology platform, the database of "medicinal herbs-ingredients" and "disease targetsingredients" of Jinlingzi san were established, screening key targets for channel enrichment analysis. Using Score ≥ 0.8 as a similarity, then the AI technology was used to analyze and interrelate the database. The multi-dimensional network analysis of "traditional Chinese medicine-component-target-pathway" for Jinlingzi powder in treating peptic ulcer was carried out. Finally the quality markers of the anti ulcer were screened and the molecular mechanism was predicted. Results By searching the database of TCM components on the platform of integrated pharmacology, seven components were collected from toosendan, mainly triterpenoids, and two potential disease targets were predicted. Thirty-one chemical components were collected from Corydalis yanhusuo, mainly alkaloids, and nine potential disease targets were predicted. Seven key targets related to the treatment of peptic ulcer, including RAC1, RAC2, NCF1, NCF2, NCF4, CYBA and ATP1A1, were screened through screening and pathway enrichment analysis. The main gene functions were vascular endothelial growth factor (VEGF) receptor signaling pathway, glutamate receptor activity and related pathways, phosphatidylinositol, platelet activation and its growth factor, Ras guanosine nucleotide exchange factor activity, etc. The database was analyzed and interlinked. finally the Glaucine was selected as the anti ulcer marker component (Q-marker). Jinlingzi san may be used to treat ulcer through nervous system, immune system, cell communication, RAS pathway, RAP1 pathway and adhesion pathway. Conclusion Jinlingzi san should study the primary target in Corydlis Rhizoma and Glaucine for further study of the anti peptic ulcer.