目的 對(duì)舌下含服和口服給予家兔治咳川貝枇杷滴丸（CBPP）后的入血成分進(jìn)行定性和定量研究，旨在明確CBPP的藥效物質(zhì)基礎(chǔ)，比較兩種給藥方式的差異。方法 UPLC-Q/TOF-MS法采用Waters ACQUITY UPLC BEH C₁₈（100mm×2.1 mm，1.7 μm）色譜柱；流動(dòng)相為乙腈（A）-0.05%甲酸水（B），梯度洗脫，體積流量為0.4 mL/min；質(zhì)譜采用正、負(fù)離子掃描，掃描范圍m/z 50~1500。舌下含服和口服兩種給藥方式分別給予家兔CBPP（200 mg/kg），給藥后于不同時(shí)間點(diǎn)耳緣靜脈取血0.5 mL，分離血漿，UPLC-Q/TOF-MS結(jié)合多元統(tǒng)計(jì)分析方法用于區(qū)分不同血漿樣本，旨在尋找對(duì)分組貢獻(xiàn)率較大的生物標(biāo)志物（Marker）即為潛在的入血成分，并通過對(duì)其峰面積進(jìn)行積分比較了兩種給藥方式下的相對(duì)含量。結(jié)果 在舌下含服和口服給藥下的血漿中共解析出6種入血成分，定量結(jié)果表明該6種成分在體內(nèi)達(dá)到最高值的時(shí)間均在1 h以內(nèi)，且在各個(gè)時(shí)間點(diǎn)下舌下含服的血藥濃度均大于口服給藥。結(jié)論 從藥效物質(zhì)基礎(chǔ)層面證明了舌下含服優(yōu)于口服給藥，為CBPP的進(jìn)一步臨床應(yīng)用與發(fā)展提供依據(jù)。

Objective To carry out the qualitative and quantitative analysis of the components in plasma after oral and sublingual administration of Zhikechuanbeipipa dropping pills (CBPP).Methods An Waters ACQUITY UPLC BEH C₁₈ (100 mm×2.1 mm, 1.7 μm) was used with 0.05% formic acid aqueous solution-acetonitrile as mobile phases by gradient elution. The metabolites were detected by electrospray ion (ESI) source in both positive and negative mode mode. Rabbits were dosed with CBPP (200 mg/kg) by oral and sublingual administration Blood samples of 0.4 mL were taken from auricular vein at different time points and plasma components and contents were determined by UPLC-Q/TOF-MS.Results A total of 6 components were identified in rabbit plasma, and the peak areas of all the 6 components in sublingual administration were higher than oral administration.Conclusion Sublingual administration is better than oral administration based on the material basis of CBPP, which will provide a basis for further clinical application and development of CBPP.