18(100mm×2.1 mm,1.7 μm)色譜柱;流動相為乙腈(A)-0.05%甲酸水(B),梯度洗脫,體積流量為0.4 mL/min;質譜采用正、負離子掃描,掃描范圍m/z 50~1500。舌下含服和口服兩種給藥方式分別給予家兔CBPP(200 mg/kg),給藥后于不同時間點耳緣靜脈取血0.5 mL,分離血漿,UPLC-Q/TOF-MS結合多元統(tǒng)計分析方法用于區(qū)分不同血漿樣本,旨在尋找對分組貢獻率較大的生物標志物(Marker)即為潛在的入血成分,并通過對其峰面積進行積分比較了兩種給藥方式下的相對含量。結果 在舌下含服和口服給藥下的血漿中共解析出6種入血成分,定量結果表明該6種成分在體內達到最高值的時間均在1 h以內,且在各個時間點下舌下含服的血藥濃度均大于口服給藥。結論 從藥效物質基礎層面證明了舌下含服優(yōu)于口服給藥,為CBPP的進一步臨床應用與發(fā)展提供依據。;Objective To carry out the qualitative and quantitative analysis of the components in plasma after oral and sublingual administration of Zhikechuanbeipipa dropping pills (CBPP).Methods An Waters ACQUITY UPLC BEH C18 (100 mm×2.1 mm, 1.7 μm) was used with 0.05% formic acid aqueous solution-acetonitrile as mobile phases by gradient elution. The metabolites were detected by electrospray ion (ESI) source in both positive and negative mode mode. Rabbits were dosed with CBPP (200 mg/kg) by oral and sublingual administration Blood samples of 0.4 mL were taken from auricular vein at different time points and plasma components and contents were determined by UPLC-Q/TOF-MS.Results A total of 6 components were identified in rabbit plasma, and the peak areas of all the 6 components in sublingual administration were higher than oral administration.Conclusion Sublingual administration is better than oral administration based on the material basis of CBPP, which will provide a basis for further clinical application and development of CBPP."/>

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首頁 > 過刊瀏覽>2019年第42卷第1期 >2019,42(1):105-110. DOI:10.7501/j.issn.1674-6376.2019.01.015
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基于UPLC-Q/TOF-MS技術的治咳川貝枇杷滴丸在家兔體內的定性定量研究

Qualitative and quantitative analysis in rabbit after oral and sublingual administration of Zhikechuanbeipipa dropping pills by UPLC-Q/TOF-MS

發(fā)布日期:2019-01-31
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