目的 觀察銀杏內(nèi)酯B （GGB）注射液對(duì)SD大鼠生育力及早期胚胎發(fā)育毒性。方法 SD大鼠隨機(jī)分為GGB注射液低、中、高劑量（30、60、120 mg/kg，分別相當(dāng)于臨床等效劑量的52、105、210倍）組及溶媒對(duì)照組（生理鹽水），每組雌雄各25只。每天尾靜脈推注給藥1次，雄鼠連續(xù)給藥4周，雌鼠連續(xù)給藥2周，以1：1合籠交配，雄鼠繼續(xù)給藥至交配成功，雌鼠繼續(xù)給藥至妊娠第7天。在確定雌鼠受精后處死同籠的雄鼠，解剖檢查睪丸和附睪等生殖器官及精子的數(shù)量、活力和形態(tài)。雌鼠妊娠第14天處死，解剖檢查卵巢、子宮和胚胎的情況。結(jié)果 雄性大鼠GGB注射液高劑量組給藥2.5、3.0、3.5、4.0周體質(zhì)量與溶媒對(duì)照組比較顯著增高（P<0.05），雄性大鼠給藥第2周中、高劑量組攝食量與溶媒對(duì)照組比較顯著增高（P<0.05、0.01），給藥第3周高劑量組攝食量與溶媒對(duì)照組比較亦顯著增高（P<0.01），攝食量的增高與體質(zhì)量的顯著性增長(zhǎng)呈現(xiàn)出較好的一致性，說(shuō)明供試品對(duì)體質(zhì)量的影響跟飼料的攝入有關(guān)。GGB注射液對(duì)雄性大鼠的睪丸、附睪的質(zhì)量及臟器系數(shù)、精子數(shù)量、精子活力、精子形態(tài)沒(méi)有顯著影響；對(duì)雌鼠的體質(zhì)量、攝食、孕鼠的黃體、活胎、死胎、吸收胎、著床數(shù)、交配率、受孕率、著床前丟失率和著床后丟失率沒(méi)有顯著影響。結(jié)論 在本試驗(yàn)條件下，未見GGB注射液對(duì)大鼠的生育力及早期胚胎的發(fā)育有毒性作用。

Objective To study the toxicity effect of Ginkgolide B (GGB) Injection on fertility and early embryonic development in rats.Methods A total of 100 male and 100 female SD rats were randomly divided into four groups:low,medium and high dosage groups of GGB Injection (30,60 and 120 mg/kg,52,105 and 210 times of clinical equivalent dose,respectively) and solvent control group (normal sodium).There were 25 male and 25 female rats in each group.The rats were given by caudal vein injection (l mL/100 g) once daily;The male rats were received successive administration for 28 days before mating and the successive administration of 14 days before mating to 7 days after pregnancy for female rats.When the female rats were confirmed to be pregnant,the male rats were executed and their testicle,epididymis and sperm were examined.The female rats were executed on the fourteenth day of pregnancy,and The ovary,uterus and embryo of the female rats were studied.Results To male rats,there was a significant increasing difference for the treatment group (120 mg/kg) compared with the control group in body weight gain of male rats in 2.5,3,3.5,and 4 week (P<0.05),and the food intake of male rats in medium and high dose group was significantly higher than that in solvent control group at the second week (P<0.05,0.01),and the food intake of high dose group was also significantly higher than that in solvent control group at the third week (P<0.01).The body weight gain may caused by food intake.There were no significant differences in mass and organ coefficients of testis and epididymis,sperm count,sperm viability,and sperm form.To female rats,there were no significant differences in the body weight,food intake,pregnancy rate,the number of corpus luteum,live embryo,dead embryo and absorbed embryo,implantation,mating rate,and the rate of lost oosperm before and after implantation.Conclusion Within the range of experiment doses,there is no significant toxic effect of GGB Injection on the fecundity and the early developme of embryo.