目的 建立同時測定黃芪中5種黃酮類成分含量的高效液相色譜（HPLC）分析方法并進行黃芪的指紋圖譜研究。方法 采用HPLC-DAD，Waters SymmetryShield^TM RP₁₈色譜柱（250 mm×4.6 mm，5 μm），乙腈（A）-水（0.05%磷酸）（B）為流動相梯度洗脫，體積流量1.0 mL/min，檢測波長246、206 nm，柱溫25℃，進樣量20 μL，對黃芪中毛蕊異黃酮葡萄糖苷、芒柄花苷、（6aR，11aR）-9，10-二甲氧基紫檀烷-3-O-β-D-葡萄糖苷、毛蕊異黃酮、7-羥基-4'-甲氧基異黃酮進行含量測定。結(jié)果 方法學(xué)考察結(jié)果顯示，黃芪中5種黃酮類成分線性范圍，相關(guān)系數(shù)良好（r=0.999 8~0.999 9，n=9），各色譜峰間分離度均達到定量要求。精密度（RSD<1.86%），穩(wěn)定性（RSD<2.01%），重復(fù)性（RSD<1.97%），加樣回收率（RSD<2.90%，n=9）等參數(shù)均達到定量分析要求。建立了黃芪的HPLC指紋圖譜，標(biāo)定了27個共有峰，樣品相似度在0.808~0.971，存在一定差異。結(jié)論 本實驗建立的方法準(zhǔn)確、簡便、重復(fù)性好，可為黃芪藥材的質(zhì)量控制提供參考。

Objective To establish a method to determine the components of five flavonoids in Radix Astragali and built fingerprint of Radix Astragali by HPLC. Methods HPLC-DAD method was used to determine five flavonoids simultaneously, including calycosin-7-glucoside, ononin, (6aR, 11aR)-9, 10-dimethoxypterocarpan-3-O-β-D-glucoside, calycosin and 7-hydroxyl-4'-methoxyisoflavone. The separation was carried on a Waters Symmetry Shield^TM RP₁₈ column (250 mm×4.6 mm, 5 μm) with the mobile phase consisting of acetonitrile (A)-water (0.05% phosphoric acid) (B) by using gradient elution. The flow rate was 1.0 mL. min^-1 and the detection wavelengths were at 246 and 206 nm. The column temperature was set at 25℃ and the injection volume was 20 μL.Results Methodology examination showed that the five flavonoids showed a good linear relationship (r=0.999 8-0.999 9, n=9), precision (RSD < 1.86%), stability (RSD < 2.01%) and repeatability (RSD < 1.97%) conformed to requirements. The fingerprint of Radix Astragali was built. 27 mutual peaks were labeled and the similarity was more than 0.808. Conclusion The established method is accurate, simple and reproducible, which can provide reference for quality control of Radix Astragali.

國家自然科學(xué)基金資助項目（21502142）