目的 觀察那如三味片（Naru-3）對(duì)創(chuàng)傷性脊髓損傷大鼠炎癥作用的影響，并探討相關(guān)機(jī)制。方法 SD大鼠隨機(jī)分為假手術(shù)組、模型組、甲基潑尼松龍（MP，30 mg/kg）組和Naru-3低、中、高劑量（10、20、30 mg/kg）組，每組12只。假手術(shù)組僅進(jìn)行手術(shù)暴露，不進(jìn)行打擊；其他組別采用Allen’s打擊法建立大鼠脊髓損傷模型。術(shù)后30 min各組ig給藥，假手術(shù)組和模型組ig等體積生理鹽水，每天1次，連續(xù)21 d。采用實(shí)時(shí)熒光定量PCR（qRT-PCR）和western blotting法檢測(cè)脊髓組織中白介素（IL）-1β、IL-6和腫瘤壞死因子-α（TNF-α）mRNA和蛋白的相對(duì)表達(dá)量；ELISA法檢測(cè)血清中IL-1β、IL-6和TNF-α蛋白含量；免疫組織化學(xué)法確定脊髓組織單核細(xì)胞趨化蛋白-1（MCP-1）定位及表達(dá)；流式細(xì)胞術(shù)分析脊髓組織M1及M2表型巨噬細(xì)胞比例。結(jié)果 與模型組比較，Naru-3、MP顯著減少血清中IL-1β、IL-6和TNF-α蛋白含量，顯著減少脊髓組織IL-1β、IL-6和TNF-α mRNA、蛋白水平（P<0.05、0.001），Naru-3作用呈劑量、時(shí)間相關(guān)性；免疫組織化學(xué)結(jié)果顯示，與模型組比較，Naru-3、MP可以明顯減少脊髓中MCP-1表達(dá)；流式細(xì)胞術(shù)結(jié)果表明，與模型組比較，Naru-3、MP顯著誘導(dǎo)M1表型巨噬細(xì)胞分化為M2表型（P<0.001）。結(jié)論 Naru-3可以有效降低脊髓損傷引起的炎癥反應(yīng)，機(jī)制與誘導(dǎo)M1表型巨噬細(xì)胞分化為M2表型相關(guān)。

Objective To observe the anti-inflammation effect of the Mongolian medicine narru-3 (Naru-3) prescription in the treatment of spinal cord injury, and explore its related mechanism. Methods SD rats were randomly divided into sham group, model group, methylprednisolone (MP, 30 mg/kg) group and Naru-3 low, medium and high dose (10, 20, 30 mg/kg) group, with 12 rats in each group. The sham group was exposed to surgery only, but not attacked. The other groups were used Allen's attacking method to establish rat spinal cord injury model. Rats were ig administered in each group 30 min after operation, and rats in sham and model group was administered with normal saline once a day for 21 d. The relative expression levels of interleukin (IL)-1β, IL-6 and tumor necrosis factor-alpha (TNF-α) in spinal cord tissues were detected by real-time fluorescence quantitative PCR (qRT-PCR) and Western blotting. The levels of IL-1β, IL-6 and TNF-α in serum were detected by ELISA. The localization and expression of monocyte chemoattractant protein-1 (MCP-1) in spinal cord tissues were determined by immunohistochemistry. The proportion of M1 and M2 phenotypic macrophages in spinal cord tissue was analyzed. Results Compared with model group, Naru-3 and MP significantly reduced the levels of IL-1β, IL-6 and TNF-α protein in serum and the mRNA and protein levels of IL-1β, IL-6 and TNF- α in spinal cord tissue (P<0.05, 0.001). The effect of Naru-3 was dose-dependent and time-dependent. Immunohistochemical results showed that Naru-3 and MP could significantly reduce the expression of MCP-1 in spinal cord compared with the model group. The results of flow cytometry showed that, compared with model group, Naru-3 and MP significantly induced M1 phenotype macrophages to differentiate into M2 phenotype (P<0.001).Conclusion Naru-3 can effectively reduce the inflammatory response induced by spinal cord injury, and the mechanism is related to inducing M1 phenotype macrophages to differentiate into M2 phenotype.

廣東省科技項(xiàng)目（2013A032500010）