目的 研究沉香醇提物對(duì)大鼠氣滯血瘀心肌缺血損傷的保護(hù)作用及機(jī)制。方法 大鼠隨機(jī)分成6組：對(duì)照組、模型組、野生沉香醇提物（2.84 g/kg）組和通體沉香醇提物低、中、高劑量（0.71、1.42、2.84 g/kg）組，每天1次，連續(xù)ig給藥7 d，對(duì)照組和模型組ig等體積蒸餾水。除對(duì)照組外，采用sc異丙腎上腺素（ISO）聯(lián)合冰水浸泡誘導(dǎo)大鼠氣滯血瘀心肌缺血模型，檢測(cè)生化指標(biāo)全血黏度（WBV）、血漿比黏度（PSV），血清中肌酸激酶（CK）、乳酸脫氫酶（LDH）、丙氨酸轉(zhuǎn)氨酶（ALT）和天冬氨酸轉(zhuǎn)氨酶（AST）活性；觀察心電圖及心肌病理?yè)p傷情況；檢測(cè)血漿中脂質(zhì)過(guò)氧化物（LPO）、過(guò)氧化氫（H₂O₂）、總抗氧化能力（T-AOC）和過(guò)氧化氫酶（CAT）的水平；酶聯(lián)免疫法（ELISA）檢測(cè)血清中細(xì)胞因子5-羥色胺（5-HT）、內(nèi)皮素（ET）、血栓烷素B2（TXB₂）及前列腺素E₂（PGE₂）的分泌水平。結(jié)果 與模型組比較，通體沉香醇提物各劑量組均能顯著降低WBV和PSV，改善心電圖的異常，顯著降低心電圖ST段的抬高（P<0.05、0.01）；中、高劑量組顯著降低血清中心肌酶CK、LDH、ALT和AST活性（P<0.05、0.01、0.001）；各劑量組明顯減輕心肌組織的病理?yè)p傷程度；高、中劑量組均顯著降低LPO和H₂O₂水平，升高T-AOC和SOD水平（P<0.05、0.01、0.001）；高劑量組顯著降低5-HT水平，各劑量組均顯著降低ET、TXB₂和PEG₂水平（P<0.05、0.01、0.001）。同等劑量下通體沉香醇提物與野生沉香醇提物作用相當(dāng)。結(jié)論 通體沉香醇提物對(duì)大鼠氣滯血瘀心肌缺血有保護(hù)作用，其作用機(jī)制與抗氧化、改善血管微循環(huán)、調(diào)節(jié)內(nèi)皮細(xì)胞生長(zhǎng)及調(diào)節(jié)細(xì)胞因子的分泌有關(guān)。

Objective To investigate the cardioprotective effect and its mechanism of agarwood alcohol extract on qi stagnation blood stasis myocardial ischemia injury in rats. Methods Rats were randomly divided into six groups:control group, model group, wild agarwood ethanol extract (2.84 g/kg) group and low, medium and high dose (0.71, 1.42, 2.84 g/kg) groups of agarwood alcohol extract produced by whole-tree agarwood-inducing technique (WTAAE). Rats were ig administrated corresponding drug once a day for 7 d. Rats in control and model group were ig administrated with distilled water of equal volume. Myocardial ischemia injury rat model was established by injecting with isoproterenol (ISO) and stimulated with ice water. The whole blood and plasma specific viscosity (WBV and PSV) were measured by viscometer. The activities of creatine kinase (CK), lactic dehydrogenase (LDH), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured in serum. Electrocardiograph (ECG) and myocardial pathological injury were observed. The plasma levels of lipid peroxide (LPO), hydrogen peroxide (H₂O₂), total antioxidant capacity (T-AOC) and catalase (CAT) were measured. The secreted levels of 5-hydroxyptamine (5-HT), endothelin (ET), thromboxane B₂ (TXB₂) and prostaglandin E₂ (PGE₂) were measured by enzyme-linked immunosorbent assay (ELISA). Results Compared with model group, WTAAE could significantly reduce WBV and PSV, improve ECG abnormalities, and significantly reduce ST segment elevation (P<0.05, 0.01); The activities of serum myosin CK, LDH, ALT and AST were significantly decreased in the middle and high dose groups (P<0.05, 0.01 and 0.001); The pathological damage degree of myocardium was significantly reduced in each dose group; LPO and H₂O₂ levels were significantly decreased in the middle and high dose group, and T-AOC and SOD levels were increased (P<0.05, 0.01 and 0.001); 5-HT levels were significantly decreased in the high dose group, and ET, TXB₂ and PEG₂ levels were significantly decreased in each dose group (P<0.05, 0.01 and 0.001). At the same dosage, WTAAE had the same effect as that of wild agarwood. Conclusion WTAAE shows a significantly protective effect on myocardial ischemia in rats, and its mechanism is related to the antioxidant activity, regulation of vascular microcirculation, endothelial growth and cytokines secretion.

海南省自然科學(xué)基金（817290）；海南省重大科技計(jì)劃項(xiàng)目（ZDKJ2016004）；海南省自然科學(xué)基金（217291）