目的 觀察辣木葉及其復方對便秘模型大鼠的通便作用和相關胃腸激素水平的影響。方法 按體質(zhì)量隨機將100只SD大鼠分為10組，即對照組、模型組、番瀉葉浸液組（陽性對照，0.01 g/kg）和辣木葉水提液低、中、高劑量（0.79、1.58、3.15 g/kg）組，黃芪白術（1.05 g/kg）組，辣木葉復方水提液低、中、高劑量（1.84、2.63、4.20 g/kg）組。除對照組外，均采用鹽酸洛哌丁胺（6.67 mg/kg）復制便秘模型，每日2次，連續(xù)2周。造模結(jié)束后，各給藥組分別ig相應受試樣品，每天1次，連續(xù)7 d。記錄各組大鼠體質(zhì)量、最后24 h糞便粒數(shù)，測定糞便含水量、小腸炭末推進率，ELISA試劑盒法檢測血清P物質(zhì)、生長抑素含量及結(jié)腸胃動素、血管活性腸肽含量。結(jié)果 與模型組比較，辣木葉水提液中、高劑量組及辣木葉復方水提液各劑量組的體質(zhì)量增量顯著升高（P<0.05）；各給藥組大鼠的排便粒數(shù)與模型組比較均顯著性增加（P<0.05）；各給藥組大鼠糞便含水量與模型組比較均無顯著性差異；辣木葉水提液中、高劑量組及其復方水提液中、高劑量組小腸炭末推進率顯著增加（P<0.05、0.01）。辣木葉水提液中、高劑量組及其復方水提液各劑量組血清P物質(zhì)含量、結(jié)腸組織胃動素含量均顯著高于模型組（P<0.05、0.01）；辣木葉水提液高劑量組及辣木葉復方水提液中、高劑量組大鼠血管活性腸肽含量較模型組降低顯著（P<0.05、0.01）；各給藥組大鼠血清生長抑素水平均顯著低于模型組（P<0.01）。辣木葉復方水提物上述作用均好于等劑量辣木葉水提液及黃芪白術水提液。結(jié)論 辣木葉及其復方具有良好的通便作用，可能與調(diào)節(jié)胃腸激素水平有關；復方的通便及調(diào)節(jié)胃腸激素的作用優(yōu)于單味藥辣木葉及黃芪白術，體現(xiàn)出復方配伍的整體優(yōu)越性，其中辣木葉側(cè)重調(diào)節(jié)P物質(zhì)、胃動素及血管活性腸肽水平，而黃芪白術主要調(diào)節(jié)生長抑素水平。

Objective To observe the impacts of Moringa oleifera and Moringa oleifera Purgative Compound on defecation and gastrointestinal hormones in rats with constipation. Methods A total of 100 SD rats with half male and half female were randomly divided into ten groups:control group, model group, Senna leaf extract group (positive control, 0.01 g/kg), Water extract of Huangqi Baizhu group, water extract of M. oleifera (WEMO) low, medium, high dose (0.79, 1.58, and 3.15 g/kg) groups and water extract of M. oleifera Purgative Compound (WEMOPC) low, middle, high dose (1.84, 2.63, and 4.20 g/kg) groups. Loperamide hydrochloride was administered at a dose of 6.67 mg/kg per day by ig for continuous two weeks so that constipation model was established. Each group was treated with corresponding drugs respectively, once a day for seven days. After one week, body weight, fecal number and water content within 24 h, intestinal propulsive rate, contents of motilin, substance P, vasoactive intestinal peptide and somatostatin were measured. Results Compared with the model group, the body weight increment of WEMO medium and high dose group and WEMOPC each dose group increased significantly (P<0.05); the fecal granule number of rats in each dose group increased significantly compared with the model group (P<0.05); the fecal water content of rats in each dose group had no significant difference compared with the model group; the small intestinal carbon powder propulsive rate of WEMO medium and high dose group and its compound water extract medium and high dose group increased significantly (P<0.05, 0.01). The contents of substance P in serum and motilin in colon tissue in WEMO medium and high dose group and its compound water extract groups were significantly higher than those in model group (P<0.05 and 0.01); The contents of vasoactive intestinal peptide in WEMO high dose group and WEMOPC medium and high dose group were significantly lower than those in model group (P<0.05 and 0.01); The levels of somatostatin in serum of rats in each treatment group were significantly lower than those in model group (P<0.01). The above effects of WEMOPC were better than those of the same dose of WEMO and water extract of Huangqi Baizhu group. Conclusion M. oleifera and its compound have good defecation effect, which may be related to the regulation of gastrointestinal hormone level. The effect of the compound on defecation and regulation of gastrointestinal hormone is better than that of the single herb M. oleifera and Huangqi Baizhu, reflecting the overall superiority of the combination. M. oleifera leaves mainly regulate the levels of substance P, motilin and vasoactive intestinal peptide, while Huangqi Baizhu mainly regulates the level of somatostatin.