目的 采用不同模型評(píng)價(jià)海馬補(bǔ)腎丸免疫調(diào)節(jié)作用和抗應(yīng)激作用。方法 健康昆明種小鼠隨機(jī)分為對(duì)照組、模型組、香菇菌多糖（100 mg/kg）組、龜齡集（0.24 g/kg）組和海馬補(bǔ)腎丸0.55、1.10、2.20 g/kg組，每天ig給藥1次，連續(xù)14 d。①采用注射鹽酸環(huán)磷酰胺致小鼠免疫抑制模型，給藥結(jié)束尾iv 25%印度墨汁，計(jì)算廓清指數(shù)（κ）和吞噬指數(shù)（α），觀察小鼠肝指數(shù)、脾指數(shù)和胸腺指數(shù)；②采用注射鹽酸環(huán)磷酰胺致小鼠免疫抑制模型，ip 5%雞紅細(xì)胞懸液0.3 mL致敏，觀察各組血清溶血素生成量；③給藥第8～10天，除對(duì)照組外，剩余各組開(kāi)始ip環(huán)磷酰胺80 mg/kg，連續(xù)3 d，繼續(xù)給藥，至給藥第14天眼眶靜脈取血，測(cè)定白細(xì)胞數(shù)及免疫球蛋白IgG、IgM、IgA含量；④SD大鼠隨機(jī)分為對(duì)照組、模型組、單硝酸異山梨酯片20 mg/kg、龜齡集0.12 g/kg和海馬補(bǔ)腎丸低、中、高劑量（0.28、0.56、1.12 g/kg）組，每天ig給藥1次，連續(xù)14 d，觀察海馬補(bǔ)腎丸對(duì)運(yùn)動(dòng)疲勞大鼠游泳時(shí)間及運(yùn)動(dòng)后對(duì)血清尿素氮（BUN）、乳酸（LD）、肝糖原水平的影響；⑤健康昆明種小鼠隨機(jī)分為對(duì)照組、模型組、單硝酸異山梨酯片（40 mg/kg）組、龜齡集（0.24 g/kg）組和海馬補(bǔ)腎丸低、中、高劑量（0.55、1.1、2.2 g/kg）組，每天ig給藥1次，連續(xù)5 d，于末次給藥后1 h，采用注射異丙腎上腺素致小鼠缺氧模型，將小鼠置于密封瓶中，觀察存活時(shí)間。結(jié)果 與模型組比較，海馬補(bǔ)腎丸2.2 g/kg顯著增加α及κ（P<0.05、0.01），海馬補(bǔ)腎丸1.1、2.2 g/kg劑量顯著增加脾、胸腺系數(shù)（P<0.05、0.01）；海馬補(bǔ)腎丸各劑量組顯著增加血清溶血素生成量（P<0.05、0.01）；海馬補(bǔ)腎丸2.2 g/kg劑量組顯著增加白細(xì)胞數(shù)量及免疫球蛋白IgG、IgM、IgA的含量（P<0.05）；海馬補(bǔ)腎丸0.56、1.12 g/kg劑量顯著延長(zhǎng)大鼠力竭游泳時(shí)間（P<0.05、0.01），1.12 g/kg劑量能顯著地減少血清中BUN、LD含量，減少肝糖原消耗（P<0.05、0.01）；海馬補(bǔ)腎丸1.1、2.2 g/kg劑量能顯著延長(zhǎng)小鼠缺氧存活時(shí)間（P<0.05、0.01）。結(jié)論 海馬補(bǔ)腎丸具有明顯免疫增強(qiáng)和抗應(yīng)激作用。

Objective Different models were used to evaluate the immunomodulatory effect and anti-stress effect of Haima Bushen Pills. Methods The healthy Kunming mice were randomly divided into control group, model group, lentinan group (100 mg/kg), Guilingji group (0.24 g/kg) and Haima Bushen Pills group (0.55, 1.10 and 2.20 g/kg). The mice were ig given relevant drugs once a day for 14 days. ① Immunosuppressive model of mice was induced by cyclophosphamide hydrochloride injection. Indian ink of 25% was iv at the end of administration. Clearance index (κ) and phagocytic index (α) were calculated. Liver index, spleen index and thymus index of mice were observed. ② Immunosuppressive model of mice was induced by injection of cyclophosphamide hydrochloride, and sensitized by 0.3 mL of IP 5% chicken erythrocyte suspension. Serum hemolysin production was observed in each group. ③ On the 8th to 10th day of administration, except the control group, the remaining groups began ip cyclophosphamide 80 mg/kg for 3 consecutive days. The orbital vein blood was collected on the 14th day of administration, and the number of white blood cells and the contents of immunoglobulin IgG, IgM and IgA were measured. ④ SD rats were randomly divided into control group, model group, isosorbide mononitrate tablets 20 mg/kg, Guilingji 0.12 g/kg and low, medium and high dose groups (0.28, 0.56, 1.12 g/kg) of Hippocampus Kidney-tonifying Pills. The effects of Hippocampus Kidney-tonifying Pills on the levels of serum urea nitrogen (BUN), lactic acid (LD) and hepatic glycogen in exercise-fatigue rats were observed once a day for 14 days. ⑤ Healthy Kunming mice were randomly divided into control group, model group, isosorbide mononitrate tablet (40 mg/kg), Guilingji (0.24 g/kg) group and Hippocampus Kidney-tonifying Pills (0.55, 1.1, 2.2 g/kg) group. The mice were given Ig once a day for 5 days. One hour after the last administration, the mice were injected with isoprenaline to induce hypoxia. The mice were placed in sealed bottles for observation of survival time. Results Compared with model group, 2.2 g/kg of Haima Bushen Pills significantly increased α and κ (P<0.05 and 0.01), 1.1 and 2.2 g/kg of Haima Bushen Pills significantly increased spleen and thymus coefficient (P<0.05 and 0.01), and each dose of Haima Bushen Pills significantly increased serum hemolysin production (P<0.05 and 0.01); 2.2 g/kg of Haima Bushen Pills significantly increased the number of white blood cells and the IgG, IgM and IgA (P<0.05); the dosage of Haima Bushen Pills (0.56, 1.12 g/kg) significantly prolonged the exhaustive swimming time of rats (P<0.05, 0.01); the dosage of 1.12 g/kg could significantly reduce the contents of BUN and LD in serum and the consumption of hepatic glycogen (P<0.05, 0.01); the dosage of Haima Bushen Pills (1.1, 2.2 g/kg) could significantly prolong the hypoxic survival time of mice (P<0.05, 0.01). Conclusion Haima Bushen Pills has obvious immune enhancement and anti - stress effect.