目的 研究注射用益氣復(fù)脈（凍干）（YQFM）對大鼠離體胸主動脈環(huán)的作用及機制。方法 采用大鼠離體胸主動脈血管環(huán)，觀察YQFM及紅參、麥冬、五味子提取物（HS、MD、WWZ）對血管的直接作用，YQFM對KCl或去甲腎上腺素所引發(fā)血管收縮作用的影響；對乙酰膽堿所致血管舒張作用的影響；對內(nèi)鈣釋放所致血管收縮作用的影響。結(jié)果 YQFM在低濃度下對血管無刺激作用，但是在高濃度時，對血管有刺激收縮作用，且無內(nèi)皮組收縮幅度明顯超出有內(nèi)皮組。不同濃度的3種藥材提取物對血管刺激作用不顯著；YQFM可顯著抑制KCl或者去甲腎上腺素引起的血管收縮；YQFM對乙酰膽堿引起的血管舒張作用無顯著抑制作用；YQFM不能抑制內(nèi)鈣釋放。結(jié)論 YQFM在高濃度下對血管有一定的直接刺激收縮作用，且直接刺激作用可能和血管內(nèi)皮相關(guān)；YQFM既有抑制受體操縱性鈣通道又有抑制電壓依賴性鈣通道的調(diào)節(jié)作用。

Objective This study explored the effect and mechanism of Yiqi Fumai Lyophilized Injection (YQFM) on the isolated thoracic aortic rings in rats.Methods The isolated thoracic aorta rings of SD rats were used to observe:the direct effects of YQFM and hongshen extract, maidong extract, wuweizi extact on the vascular rings, effect of YQFM on vasoconstriction induced by potassium chloride or phenylephrine, effect on vasodilatation induced by acetylcholine, effect of endocalcium release on vasoconstriction.Results At low concentrations, YQFM has no stimulating effect on thoracic aortic rings. At high concentrations, YQFM has a stimulating contraction effect on blood vessels and the amplitude of contraction in the non-endothelial group is significantly higher than that in the endothelial group; the effects of the three extracts on thoracic aortic rings were weak, at high concentration, the effect has no significant difference; the vasoconstriction effect induced by potassium chloride or phenylephrine was inhibited by YQFM; the vasodilatation effect induced by acetylcholine was not inhibited. YQFM could not inhibit the release of internal calcium.Conclusions At high concentration, YQFM has a certain direct stimulation and contraction effect on blood vessels, and the stimulation effect may be related to multiple components. Moreover, the vascular endothelium may be the potential target of the direct stimulation effect. Both the receptor-manipulative calcium channel and the voltage-dependent calcium channel can be inhibited by YQFM.

天津市中藥注射劑關(guān)鍵技術(shù)校企協(xié)同創(chuàng)新實驗室建設(shè)項目（17PTSYJC00090）