目的 觀察何首烏醇提物對大鼠肝臟的毒性作用，并探討其可能的毒性機制。方法 SD大鼠連續(xù)6周ig給予生、制何首烏醇提物（10、20 g生藥/kg），觀察動物一般狀況、體質(zhì)量變化；給藥結束時檢測血清生化指標，測定肝臟質(zhì)量并計算肝臟系數(shù)，進行肝臟組織病理學檢查；提取肝臟線粒體測定呼吸鏈復合物Ⅰ活性，透射電鏡觀察肝臟線粒體結構。結果 生、制何首烏醇提物連續(xù)ig給藥6周可導致大鼠出現(xiàn)腹瀉、體質(zhì)量增長緩慢等表現(xiàn)；對丙氨酸氨基轉移酶（ALT）、天門冬氨酸氨基轉移酶（AST）等生化指標和肝臟系數(shù)無明顯影響；使大鼠肝臟出現(xiàn)輕度炎細胞浸潤、肝細胞片狀壞死、纖維增生、空泡等組織病理學變化；能降低大鼠肝臟線粒體呼吸鏈復合物Ⅰ活性，透射電鏡下可見個別線粒體嵴紊亂、斷裂或消失，出現(xiàn)空泡。結論 生、制何首烏醇提物均可引起大鼠肝臟一定程度的損傷，其肝毒性機制可能與影響肝臟線粒體的結構和電子傳遞鏈功能有關。

Objective To observe the hepatotoxicity of alcohol extracts of Polygonum multiflorum in rats, and to further study the possible mechanisms of hepatotoxicity.Methods SD rats were given alcohol extracts of raw or processed Polygonum multiflorum (10, 20 g/kg) for 6 weeks, the general conditions of rats and body weight were observed. At study termination, the serum level of biochemical indexes were detected, the liver coefficient was calculated, and light microscopic observations of liver were conducted. At the same time, the activity of liver mitochondrial respiratory chain complex Ⅰ was measured and the structure of liver mitochondrion was observed by transmission electron microscope. Results Alcohol extracts of raw and processed Polygonum multiflorum both could induce diarrhea in rats and inhibit growth of rats, but biochemical indexes and liver coefficient showed no obvious changes. Mild inflammation cells infiltration, laminar necrosis of liver cells, fibroplasia and vacuoles were observed under light microscope. Compared with the control group, the activity of liver mitochondrial respiratory chain complex Ⅰ was decreased. Disruption or disappearance of mitochondrial crista were observerd under transmission electron microscope. Conclusion Alcohol extracts of raw and processed Polygonum multiflorum both could induce hepatotoxicity in rats to some degree, impairment of liver mitochondrial structure and mitochondrial respiratory chain function may be one of the mechanisms.

2017年四川省省級科研院所基本科研業(yè)務費