® C18柱(150 mm×2.1 mm,1.6 μm),乙腈-0.1%甲酸水進行梯度洗脫,體積流量為0.1 mL/min,柱溫為25℃,進樣量為2 μL,檢測波長為254 nm。結(jié)果 33批不同批次疏血通注射液中有28個共有峰,其中有15個已知峰,分別為組氨酸(His)、精氨酸(Arg)、絲氨酸(Ser)、甘氨酸(Gly)、谷氨酸(Glu)、天冬氨酸(Asp)、蘇氨酸(Thr)、脯氨酸(Pro)、丙氨酸(Ala)、酪氨酸(Tyr)、甲硫氨酸(Met)、纈氨酸(Val)、亮氨酸(Leu)、賴氨酸(Lys)、苯丙氨酸(Phe),不同批次的樣品與標準指紋圖譜相似度均在0.994以上。結(jié)論 本實驗建立的UPLC指紋圖譜分析方法穩(wěn)定、精密度高,可應用于疏血通注射液的質(zhì)量控制和評價。;Objective To establish a UPLC fingerprint analysis method for free amino acids in Shuxuetong Injection.Methods Using phenyl isothiocyanate (PITC) derivatization method, using a Waters Acquity ultra performance liquid chromatograph, gradient elution with CORTECS® C18 column (150 mm×2.1 mm, 1.6 μm) in acetonitrile-0.1% formic acid water, the flow rate is 0.1 mL/min, column temperature is 25℃, the injection volume is 2 μL, and the detection wavelength is 254 nm.Results There are 28 common peaks in 33 batches of different batches of Shuxuetong Injection, including 15 known peaks, namely histidine (His), arginine (Arg), serine (Ser), glycine (Gly), glutamic acid (Glu), aspartic acid (Asp), threonine (Thr), proline (Pro), alanine (Ala), tyrosine (Tyr), methionine (Met), valine (Val), leucine (Leu), lysine (Lys), phenylalanine (Phe), the similarity of samples and standard fingerprints of different batches are above 0.994.Conclusion The UPLC fingerprint analysis method established in this experiment is stable and has high precision. It can be applied to the quality control and evaluation of Shuxuetong Injection."/>

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首頁 > 過刊瀏覽>2019年第42卷第7期 >2019,42(7):1350-1354. DOI:10.7501/j.issn.1674-6376.2019.07.016
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疏血通注射液中游離氨基酸的UPLC指紋圖譜研究

Study on UPLC fingerprint of free amino acid in Shuxuetong Injection

發(fā)布日期:2019-07-08
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