目的 探討實(shí)驗(yàn)動(dòng)物組織冰凍切片制備的關(guān)鍵要點(diǎn)和注意事項(xiàng)，以提高切片質(zhì)量。方法 采用美國(guó)賽默飛世爾恒溫式冷凍切片機(jī)，對(duì)30例SD大鼠和30例食蟹猴的皮膚、肌肉、乳腺、卵巢、子宮、胃、大腸、甲狀腺、肝臟、腎臟、脾臟、淋巴結(jié)、腦、脂肪等新鮮組織進(jìn)行常規(guī)冰凍切片取材、包埋、切片、染色后封片。結(jié)果 鏡下檢查組織切面平整，細(xì)胞形態(tài)完整、清晰，染色良好。結(jié)論 不同組織需要區(qū)別對(duì)待，制作優(yōu)良的冰凍切片就需要對(duì)取材、組織速凍、溫度選擇、組織包埋、切片、固定等多個(gè)環(huán)節(jié)進(jìn)行嚴(yán)格謹(jǐn)慎地操作。

Objective To improve the quality of slices, we explore the key points and consideration of freezing sectioning of experiment animals. Methods A total 30 SD tats and 30 cynomolgus monkey specimens of skin, muscle, mammary gland, ovary, uterus, stomach, intestine, thyroid, liver, kidney, spleen, lymph node, brain and fat, etc. were trimmed, embedded, sectioned and staining for routine freezing sections by Thermo low temperature cryostat microtome.Results Under microscope, the sections were smooth, and the cell morphology was complete, clear and well stained. Conclusion The different tissues need to be treated differently. To make fine frozen sections, it is necessary to operate strictly and carefully, including tissues preparation, quick freezing, selection of temperature, embedding, slicing and fixing.

國(guó)家科技重大專項(xiàng)（2015ZX09501007-004）；重大新藥創(chuàng)制（2015ZX09501004-002）