用薄層刮板-熒光分析法檢測中藥復(fù)方制劑之君藥丁公藤ErycibeobtusifoliaBenth的有效成分東莨菪亭(scopoletin)的含量,克服了薄層色譜干擾,取得了準(zhǔn)確、可靠的結(jié)果。本文的研究為中藥復(fù)方制劑的質(zhì)量控制提供了一種較好的分析手段。

A thin layer chromatograph-fluorescent method was used to assay content of scopoletin in Erycibe obtusi folia Benth.. The method showed that it not only can minimize analytical errors in thin layer scan and column contamination in HPLC,but also eliminated mutual interference of components in the preparation. It provides a good assay technique for quality control of hebal preparations. The method was carried out on thin layer plates of silica gel H(40μ)with benzene-ether (1. 1: 1 )as the developer,at an excitation spectrum of 347nm,fluorescence spectrum of 420nm and a temperture of 25°±1℃.The method showed good linearity(r= 0. 9998, n = 5)in the range of 0.08~0. 40μg/μl of scopoletin and the average recovery was 98. 52 % ~ 102. 67 % with RSD 1. 5% (n = 5).