通過觀察化學(xué)發(fā)光及檢測H2O2產(chǎn)生,考察錦燈籠的主要成分酸漿苦素B(PhysalinB,PhB)對調(diào)理酵母多糖激活的多形核中性粒細胞(polymorphonuclearleukocyte,PMN)的影響。結(jié)果顯示,不同濃度的PhB對活化的PMN化學(xué)發(fā)光呈劑量相關(guān)的抑制作用,同時對活化PMNH2O2的產(chǎn)生也有相同趨勢的抑制作用。提示錦燈寵抗炎機理可能是抑制PMN氧自由基的產(chǎn)生和釋放而發(fā)揮作用。

Effect of opsonized zpoosan on polymorphonuclear leukocyte (PMN)were studied by chemilundnescence (CL)and hydrogen peroxide (H2O2)assays. Results showed that different concentrations of Physalin B(PhB) presented a dose-dependent inhibitive effect on CL and H2O2 production of activated PMN. It suggested that the anti-inflammatory action of PhB may be related to the inhibition of production and release of oxygen free radical by PMN.