從何首烏（Polygonum multiflorum Thunb.）的根中分離出11個(gè)化合物，根據(jù)光譜學(xué)分析分別鑒定為：大黃酚（chrysophanol，Ⅰ）、大黃素甲醚（physcione，Ⅱ）、大黃素（emodin，Ⅲ）、ω-羥基大黃素（citreorosein，Ⅳ）、大黃酚8-O-β-D-吡喃葡萄糖苷（chrysophanol8-O-β-D-glucopyranoside，Ⅴ）、大黃素甲醚8-O-β-D-吡喃葡萄糖苷（Physcione8-O-β-D-glucopyranoside，Ⅵ）、大黃素8-O-β-D-吡喃葡萄糖苷（emodin8-O-β-D-glucopyranoside，Ⅶ）、決明酮8-O-β-D-吡喃葡萄糖苷（torachrysone8-O-β-D-glucopyranoside,Ⅷ）、2，3，5，4′-四羥基芪2-O-β-D-吡喃葡萄糖苷（2，3，5，4′-tetrahydroxystilbene2-O-β-D-glucopyranoside，Ⅸ）、沒食子酸甲基酯（methylgallate，Ⅹ）和吲哚-3-（L-α-氨基-α-羥基內(nèi)酸）甲酯[Indole-3-（L-α-amino-α-hydroxypropionicacid）methylester]。其中，化合物Ⅺ為新的天然產(chǎn)物。上述蒽醌和萘類化合物以及蘆薈大黃素（aloe-emodin，Ⅻ）、大黃酸（rhein，）、蘆薈大黃素8-O-β-D-吡喃葡萄糖苷（aloe-emodin8-O-β-D-glucopyranoside，）、大黃酚8-O-β-D-（6′-O-丙酰基）吡喃葡萄糖苷（chrysophanol8-O-β-D-（6′-O-malonyl）glucopyranoside , XV) 、 番瀉葉昔A(sennoside A , XVI) 、 和番瀉葉昔 B(sennoside ,X)在100μmol/L 離體 條件下 , 對(duì)重組HIV-1 (Reeombinant HIV-l)蛋白酶活性無(wú)抑制作用 。

From the root of Polygonum multiflorum Thunb. a new indole derivative,named In dole- 3 (L-α-amino-a-hydroxy propionic acid)methyl ester (Ⅺ), was isolated together with the ten known compounds,chrysophanol (Ⅰ), physcione (Ⅱ), emodin (Ⅲ),citreorosein (Ⅳ), chrysophanol 8-O-β-D-glucopyranoside (Ⅴ), physcione 8-O-β-D-glucopyranoside (Ⅵ),emodin 8-O-β-D-glucopyranoside (Ⅶ), torachrysone 8-O-β-D-glucopyranoside (Ⅷ), 2, 3, 5, 4' -tetrahydroxystilbene2-O-β-Dglucopyranoside(Ⅸ),and methylgallate (Ⅹ). Their structures were determined by spectroscopic means. These anthraquinone compounds and aloe-emodin(Ⅻ),rhein(Ⅻ),aloe-emodin 8-O-β-D glucopyranoside, chrysophanol 8-O-β-D- (6'-O-malonyl)glucopyranoside , sennoside A and sennoside B had no inhibitory effect against recombinant HIV-1 protease at concentration of 100 μmol/L in vitro.