[關(guān)鍵詞]
[摘要]
采用隨機(jī)擴(kuò)增多態(tài)DNA(RAPD)技術(shù)對山麥冬屬的4種植物進(jìn)行研究,并以沿階草OphiopogonbodlinienLevl.為組外比較,用SPSS3.1+軟件對實(shí)驗(yàn)數(shù)據(jù)進(jìn)行統(tǒng)計分析,得到聚類圖。觀察所得結(jié)果,發(fā)現(xiàn)這5種植物被分為兩大類群,短葶山麥冬、闊葉山麥冬、山麥冬、湖北山麥冬為一類,沿階草為另一類,這與經(jīng)典分類相一致,前4種隸屬于山麥冬屬,后一種屬于沿階草屬;同時,我們發(fā)現(xiàn)短葶山麥冬和闊葉山麥冬相對遺傳距離較近,結(jié)果為闊葉山麥冬是短葶山麥冬的闊葉變種提供分子遺傳方面的佐證。
[Key word]
[Abstract]
Four species of Lzriope Lour. genus were studies by means of Randomized amplification of polymorphous DNA (RAPD) technique , with你hiopogon bodinieri Levl. as the outgroup reference. Analysis of the experimental data with the aid of SPSS 3. 1 revealed that the 5 plants can be classified into two genus as was done in the classical classification,i. e. L. spicata(Thunb.)Lour. ,L. spicata(Thunb.)Lour. var. prolifera Y. T. Ma,L. platyphylla Wang et Tang and L. muscari (Decne) Bailey belong to genus 1.. Lour. ,while O. bod- inieri Levl. belongs to genus D. Ker-Gravel. It was also found that L. Platyphylla was more genetically related to L. muscari,providing the evidence that it is a broad-leaf variety of L. muscari(Decne) Bailey.
[中圖分類號]
[基金項(xiàng)目]
霍英東教育基金