建立了測定離體透皮接受液中丹參酮ⅡA的HPLC測定法。流動(dòng)相：甲醇-水(85:15);紫外檢測波長254nm,流速１mL/min。實(shí)驗(yàn)表明在230~2300ｎg/ｍＬ濃度范圍內(nèi),丹參酮Ⅱ_Ａ的濃度與峰高的線性關(guān)系良好,ｒ=0.9984。皮膚滲透液中的丹參酮Ⅱ_Ａ的平均回收率為101.62%,日內(nèi)、日間 RSD分別4.45%及4.23%。該法簡便,分離完全,結(jié)果可靠,適用于丹參酮 Ⅱ_Ａ的離體透皮液濃度的測定。

HPLC method was established for the determination of Tanshinone ⅡA(Tan ⅡA) concen-tration in the Valia-Chien diffusion cell. The mobile phase consisted of methanol:water (85:15). The detected wave length was 254 nm and flow rate was 1 ml/min. The assay linearity was determined over the range of 230-2300 ng/ml in 5% PEg 600 permeability solution (r=0. 9984). The mean recovery of Tan ⅡAwas 101.62 0 o. The within day and day to dayv ariati ons were 4.45% and 4.23% respectively which were both less than 10%The method is simple,rapid, and reliable. It is suitable for the determination of Tan.ⅡAconcentration.

國家自然科學(xué)基金,39400180