RP-HPLC法對(duì)黃芪毛狀根中黃芪甲苷進(jìn)行定量分析,在實(shí)驗(yàn)條件下,黃芪甲苷標(biāo)準(zhǔn)品在 10.64~53.20μg范圍內(nèi)與峰面積呈良好的線性關(guān)系;平均加樣回收率為92.66%;精密度試驗(yàn)相 對(duì)標(biāo)準(zhǔn)偏差為4.20%和2.40%;測(cè)得黃芪毛狀根中黃芪甲苷的含量為0.14%(干重)。

An accurate HPLC method for the determination of astragaloside N in the hairy root cultures of Astragalus membranaceus (Fisch.)Bunge was developed. Calibration graphs was rectilinear between 10. 64μg and 53. 20μg under the experimental conditions. The recovery was 92. 66% The relative standard deviations (RSDs) of measurement precision test were 4. 20% and 2. 40% The astragaloside N content measured in the haiy root cultures was 0. 14% (dry weight).

國(guó)家自然科學(xué)基金;上海市科委和教委資助項(xiàng)目