體外試驗(yàn)中,采用MTT比色法測定當(dāng)歸醇沉物對小鼠脾及胸腺淋巴細(xì)胞增殖功能的影響。結(jié)果顯示,0.16～2.50 ｍg/ｍＬ范圍內(nèi)的當(dāng)歸醇沉物能單獨(dú)或協(xié)同 ConA/LPS發(fā)揮促進(jìn)小鼠脾臟及胸腺Ｔ、Ｂ淋巴細(xì)胞增殖的作用。當(dāng)歸醇沉物尚可對抗氫化潑尼松(HP)對ConA誘導(dǎo)的脾臟及胸腺Ｔ淋巴細(xì)胞的增殖反應(yīng)的抑制作用。

Sediment (ESA) obtained from the alcoholic precipitate of extract of tuber of Agelica sinensis (Oliv.)Diels in the preparation of Angelica injection was considered as a waste product. Its effect on lymphocyte proliferation of mice spleen and thymus was tested in vitro by MTT colorimetry. Results showed that at concentrations in the range of 0. 16 ~2. 50 mg/mL. ESA significantly promoted T,B lymphocyte proliferation either alone or with ConA/LPS, and can also antagonize hydroprednisone induced suppression of T lymphocyte proliferation in vitro.

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