研究了長春花冠癭細(xì)胞接種物年齡與接種量對(duì)懸浮培養(yǎng)細(xì)胞生物產(chǎn)量的影響,探討了以大麗花輪枝孢菌的勻漿物為外源刺激物處理冠癭細(xì)胞,對(duì)細(xì)胞生長和吲哚生物堿積累的作用。確立了外源刺激物產(chǎn)生效應(yīng)的最適條件。

Influences of cell age (at inoculation) and the amount inoculated on the biological mass production of cultured cells in Catharanthus roseus (L.)g. Don suspension were inves tigated. Effects of treatment by Verticillium dahliae homogenate as elicitor on the cell growth and accumulation of indole alkaloids in the crown gall cells were studied and the optimal stimulation conditions were determined.