在國(guó)內(nèi)首次研究了貫葉連翹胚軸和子葉的培養(yǎng)方法,分別通過(guò)器官型和器官發(fā)生型途徑獲得了大量再生植株,部分可移栽成活。實(shí)驗(yàn)表明,在ＢＡ和２,４-Ｄ不同組合的ＭＳ培養(yǎng)基上,愈傷組織易于誘導(dǎo),繁殖速度快,并且通過(guò)初步檢測(cè)證明了愈傷組織中金絲桃素存在的可能性。再生植株產(chǎn)生極容易,繁殖系數(shù)高。

Tissue culturing of Hypericum perforatum L. from plumular axis and cotyledon explants were studied for the first time in our country. Regenerated individual plantlet could be obtained through callus or explants directly, and some of them could be transplanted successfully. On MS medium supplemented with BA and 2,4-D, the callus wa:; easy to induce and propagated rapidly. Hypericin was possibly present in the callus by initial tests. The regenerated individual plantlets were easy to obtained and its propagate coefficient was high.