18色譜柱上,以MeOH-MeCN-H2O(37:1:62),甲酸調(diào)節(jié)pH至2.67為流動相,2組分分離完全,工作曲線線性良好、迷迭香酸在 0.661~5.510 μg、紫草酸 B在 0.601~5. 010μg范圍內(nèi)均呈線性關(guān)系,回收率分別為 100.5%(RSD 2.82%)和 100.2%(RSD 2.53%)。;A RP-HPLC method was established for the simultaneous separation and determination of rosmarinic acid and lithospermic acid B in Salvia miltiorrhiza Bunge cultures on a Novapak C18 column, employing MeOH-MeCN-H2O (37∶1∶62) adjusted to pH 2.67 with formic acid as the mobile phase. The recoveries were 100.5%, RSD 2.82% for rosmarinic acid and 100.2%, RSD 2.53% for lithospermic acid B."/>

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RP-HPLC法測定丹參培養(yǎng)物中兩種水溶性酚酸類化合物的含量

Determination of the Contents of Two Water Soluble Phenolic Acids in Den-shen (Salvia miltiorrhiza) Cultures by RP-HPLC

發(fā)布日期：2015-01-08
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    [關(guān)鍵詞]
    [摘要]
    用 RP-HPLC法對丹參培養(yǎng)物中迷迭香酸和紫草酸 B進行了定量分析。在 Nova pah C18色譜柱上,以MeOH-MeCN-H2O(37:1:62),甲酸調(diào)節(jié)pH至2.67為流動相,2組分分離完全,工作曲線線性良好、迷迭香酸在 0.661~5.510 μg、紫草酸 B在 0.601~5. 010μg范圍內(nèi)均呈線性關(guān)系,回收率分別為 100.5%(RSD 2.82%)和 100.2%(RSD 2.53%)。
    [Key word]
    [Abstract]
    A RP-HPLC method was established for the simultaneous separation and determination of rosmarinic acid and lithospermic acid B in Salvia miltiorrhiza Bunge cultures on a Novapak C18 column, employing MeOH-MeCN-H2O (37∶1∶62) adjusted to pH 2.67 with formic acid as the mobile phase. The recoveries were 100.5%, RSD 2.82% for rosmarinic acid and 100.2%, RSD 2.53% for lithospermic acid B.
    [中圖分類號]
    [基金項目]
    上海市衛(wèi)生局和上海市教委資助
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