用薄層掃描法測(cè)定施康復(fù)顆粒劑 (Ⅱ)中苦參堿的含量。用甲苯 -丙酮 -乙醇 -濃氨試液 (2 0: 2 0: 3:1)為展開劑,在硅膠 G薄層板上分離苦參堿,以改良碘化鉍鉀為顯色劑,采用雙波長(zhǎng)反射法鋸齒掃描測(cè)定其含量,λS=5 15 nm,λR=6 5 0 nm,結(jié)果表明,苦參堿在 2 .32 8～ 6 .984μg范圍內(nèi)呈線性關(guān)系,回歸方程為 Y=2 886 .0 6 X - 2 0 6 6 .96,r=0 .9980。加樣回收率為 97.7%,RSD =2 .31%。本法簡(jiǎn)便、快速

A TLC method for the quantitation of matrine in Shikangfu granules Ⅱ was described. A mixed solvent of toluene-acetone-ethanol-concentrated ammonia solution (20:20:3:1) was employed as the developer, and dected at 515 nm. Colour developing agent was modified bismuth potassium iodide. Results gave a linear concentration range of 2.328～6.984 μg, regression equation of Y=2 886.02～2 066.96, correlation coefficient r=0.998 0 and average recovery and RSD 97.7% and 2.31%. This method is simple, relatively fast and reproducible.