應(yīng)用體外集落與液體培養(yǎng)、細(xì)胞形態(tài)學(xué)觀察、DNA片段凝膠電泳及 DNA片段百分率測(cè)定等方法觀察靈芝復(fù)方對(duì)人骨髓 CFU- GM生長(zhǎng)及 HL- 6 0細(xì)胞增殖和凋亡的作用。發(fā)現(xiàn)靈芝復(fù)方在一定濃度范圍內(nèi)對(duì)人骨髓 CFU - GM生長(zhǎng)有促進(jìn)作用,而對(duì)白血病細(xì)胞系 HL - 6 0細(xì)胞集落生長(zhǎng)則表現(xiàn)出劑量依賴性抑制 ;細(xì)胞形態(tài)學(xué)結(jié)果表明,靈芝復(fù)方對(duì) HL- 6 0細(xì)胞凋亡具有劑量和時(shí)間依賴性誘導(dǎo)作用。 8和 16 mg/ m L 靈芝復(fù)方作用48h,可使 HL - 6 0細(xì)胞 DNA片段百分率明顯升高 ;DNA片段凝膠電泳顯示有明顯的細(xì)胞凋亡“梯形”表現(xiàn)。提示靈芝復(fù)方可通過(guò)選擇性誘導(dǎo)白血病細(xì)胞凋亡來(lái)發(fā)揮抗白血病作用

A series of experiments including semi-solid colony culture and liquid culture in vitro, DNA fragments in gel electrophoresis and the percentage of DNA fragmentation test were undertaken to investigate the effects of Ganoderma lucidum (Leyss ex Fr) Karst Compound (GLC) on the growth of CFU-GM derived from human bone marrow and on the proliferation, apoptosis of HL-60 cells. The results indicated that different concentrations of GLC could promote human BM CFU-GM proliferation, but suppressed the growth of HL-60 cell colonies. The cell morphological observation discovered that GLC induced HL-60 cells apoptosis in a dose-dependent and time-dependent manner. Treating HL-60 cells with 8 mg/mL or 16 mg/mL GLC for 48 h increased the percentage of DNA fragmentation of these cells, and produced typical ladders of DNA fragments in gel electrophoresis. It is concluded that GLC could kill the leukemic cells by inducing them to apoptosis. GLC would be a good medicine for leukemia therapy.

湖南省衛(wèi)生廳基金