[關(guān)鍵詞]
[摘要]
用西洋參作 DNA的供體,用栽培大豆品種作為受體,利用分子育種導(dǎo)入技術(shù)之一的“浸種法”進(jìn)行外源 DNA導(dǎo)入,試圖在繼續(xù)保持大豆?fàn)I養(yǎng)價(jià)值和具有的醫(yī)療保健作用的前提下,提高其已有的藥用成分的含量和導(dǎo)入新的藥用成分,培育出具更高藥用價(jià)值或保健作用的栽培大豆新材料和新品系。目前導(dǎo)入后代在形態(tài)、品質(zhì)和藥物成分含量等性狀方面產(chǎn)生了廣泛的變異。本文報(bào)道了導(dǎo)入后代形態(tài)上的變異
[Key word]
[Abstract]
Soybean, besides its high nutritional value, has been long known to contain saponins and isoflavones with medicinal activites. For the purpose to increase the existing nutrients and to impart certain new constituents with pharmacologic actions, experiments for its genetic transformation by the "seed soaking method" were carried out since 1994. American ginseng, Panax quinquefolium L., was used as the DNA donor and soybean "Xiangchundou 10" as the DNA acceptor. Results showed that the transformed rate by the "seed-soaking method" was 22.6%. The transformed off spring showed morphologic and seed quality variations in mature stage, plant height, number of pods per plant, weight per 100-seeds, leaflet and seed shape, color of seed, pod, and villi, and grain protein content, etc.. The results indicated that "seed-soaking method" can transfer foreign DNA into the recipient and induce variations. The results also indicated that the method is very effective, simple, and economical, and may be used to create new germplasms and realize gene exchangement of different species, genera, and families.
[中圖分類號(hào)]
[基金項(xiàng)目]