對(duì)“大承氣湯”顆粒進(jìn)行了定性定量研究。通過薄層色譜法鑒別大黃、厚樸、枳實(shí) ;反相高效液相色譜法測定方中君藥大黃游離型蒽醌和總蒽醌的含量,采用 ODS(3)5μm色譜柱,4.6mm×250 mm,流動(dòng)相:甲醇-1%高氯酸水溶液(85∶15),流速為1.0mL/min,檢測波長:254nm;測定回收率:游離型蒽醌以大黃素、大黃酚為指標(biāo),回收率為101.97%,RSD=3.44%,總蒽醌以大黃酸、大黃素、大黃酚、大黃素甲醚為指標(biāo),回收率為98.99%,RSD=1.53%。測定結(jié)果:3批樣品的平均含量,游離型蒽醌為0.0303mg/g,總蒽醌為13.614mg/g。

The identity and contents of Radix et Rhizoma Rhei,Cortex Magnoliae Off icinalis and Fructus Aurantii Immaturus in Dachengqitang Granule were determined by TLC.The “ monarch”ingredients including free anthraquinones and total anthraquinones were determined by RP-HPLC.The average recovery rate of free anthraquinones (emodin and chrysophanol) was 101.97%,RSD= 3.44% ;and that of total anthraquinones (rhein,physcion,emodin,etc.) was 98.99%,RSD= 1.53%.