用HPLC法測定4種不同工藝制備的黃連解毒湯提取物粉末及2種日本漢方顆粒劑黃連解毒湯中的小檗堿和巴馬汀含量。結(jié)果表明此方法簡便、快速、精密度高、回收率較好。采用此法比較了上述6種黃連解毒湯的生物堿含量。結(jié)果是新工藝制備的提取物中生物堿含量最高,其中小檗堿含量分別是東洋、津村黃連解毒湯的6倍和13.8倍,是傳統(tǒng)水煎提取物的3倍。

Contents of berberine and palmatine in four powdery preparations from “ Antidotal Decoction of Compounded Coptis” and two granular Japanese Kampo “Oren-gedoku-to” were determinated by HPLC.Results showed that powdery preparation obtained by the new production process showed a higher content of the two alkaloids than the two Japanese Kampo 6～13.8times and about 3 times of the products obtained from the traditional water decoction process.The HPLC determination was shown to be simple,rapid,with high precision and satisfactory recovery.