體外培養(yǎng)大鼠皮質(zhì)神經(jīng)元,用β-淀粉樣蛋白(β-AP)誘導(dǎo)皮質(zhì)神經(jīng)元凋亡。黑海參多糖使皮質(zhì)神經(jīng)元存活率增加,使神經(jīng)元的DNA電泳圖譜不出現(xiàn)“梯子狀”,使神經(jīng)元的流式細(xì)胞儀分析圖上不出現(xiàn)凋亡峰,表明黑海參多糖對(duì)β-AP誘導(dǎo)的皮質(zhì)神經(jīng)元凋亡具有保護(hù)作用。

Cortical neurons were cultured in vitro and induced to apoptosis by amyloid β-protein (β-AP).Polysaccharides from several TCM were screened with the apoptotic model.It was found that the polysaccharide obtained from Holothuria atra Jaegar increased the survival rate of cortical neurons in a dose dependent manner,and prevented the neuronal nuclei from shrinkage,condensation and cleavage induced by β-AP.It also prevented neuronal nuclear DNA fragmentation and blocked the neuronal apoptotic peak induced by β-AP.These results indicated that polysaccharide from H.atra can block cortical neuronal apoptosis induced byβ-AP.

由國(guó)家杰出青年基金(No.396250022);國(guó)家自然科學(xué)基金(No.39770851 No.39870265);廣東省自然科學(xué)基金(No.960125)