從日本七葉樹AesculusturbinataBl.種子的90%乙醇提取物中分得3個(gè)二萜皂化合物,經(jīng)光譜學(xué)分析鑒定了它們的結(jié)構(gòu)分別為:七葉樹皂苷IVc:22α-O-巴豆?；?28-O-乙?；呷~樹皂苷配基-3β-O-[β-D-吡喃葡萄糖基(1→2)][β-D-吡喃葡萄糖基(1→4)]-β-D-吡喃葡萄糖醛酸()、異七葉樹皂苷Ia:21β-O-巴豆?；?28-O-乙?；呷~樹皂苷配基-3β-O-[β-D-吡喃葡萄糖基(1→2)][β-D-吡喃葡萄糖基(1→4)]-β-D-吡喃葡萄糖醛酸()和異七葉樹皂苷Ib:21β-O-當(dāng)歸?；?28-O-乙?；呷~樹皂苷配基-3β-O-[β-D-吡喃葡萄糖基(1→2)][β-D-吡喃葡萄糖基(1→4)]-β-D-吡喃葡萄糖醛酸(),均為首次從日本七葉樹種子中分得。七葉樹皂苷Ia、Ib、IVc和異七葉樹皂苷Ia、Ib在100μmol/L濃度的體外試驗(yàn)中對HIV-1蛋白酶活性具有一定的抑制作用

Three triterpenoid saponins were isolated from 90% ethanolic extract of the seeds of Aesculus turbinata Bl Their structures were identified on the basis of spectral data as escin IVc:22α O tigloyl 28 O acetylprotoaescigenin 3β O [β D glucopyranosyl(1→2)][β D glucopyranosyl(1→4)] β D glucopyranosiduronic acid (Ⅰ), isoescin Ia:21β O tigloyl 28 O acetylprotoaescigenin 3β O [β D glucopyranosyl(1→2)][β D glucopyranosyl(1→4)] β D glucopyranosiduronic acid (Ⅳ), and isoescin Ib:21β O angeloyl 28 O acetylprotoaescigenin 3β O [β D glucopyranosyl(1→2)][β D glucopyranosyl(1→4)] β D glucopyranosiduronic acid (Ⅴ) Escin IVc and isoescins Ia, Ib were obtained for the first time from this plant Escins Ia, Ib, IVc and isoescins Ia, Ib had inhibitory effect against recombinant HIV 1 protease at concentration of 100 μmol/L in vitro

國家自然科學(xué)基金資助項(xiàng)目!No 2 9972 0 0 4