以角叉菜膠(Car)25mg/kgsc誘導大鼠氣囊滑膜炎為模型,分別采用Lowry法、試管稀釋法、硫代巴比妥酸分光光度法,觀察了三七總皂苷(PnS)對氣囊滑膜炎滲出液中蛋白含量、白細胞數量及脂質過氧化物丙二醛(MDA)含量的影響,此外,采用細胞色素C還原法及放射免疫分析法,進一步觀察了PnS對滲出液中中性粒細胞(Neu)釋放超氧陰離子(O÷2)及其胞內cAMP含量的影響。結果發(fā)現:PnS60,120,240mg/kg3個劑量組均能劑量依賴性地抑制Car誘導的白細胞游出和蛋白滲出;顯著降低MDA含量、抑制Neu釋放O÷2;但能升高Neu內cAMP含量。提示PnS對大鼠氣囊滑膜炎具有明顯抗炎作用,其抗炎機制與升高Neu內cAMP從而抑制氧自由基生成、減輕脂質過氧化損傷有密切關系

Effects of saponin of Panax pseudo ginseng var notoginseng (Burk ) Hoo et Tseng (PnS) on neutrophil (Neu) count, protein and malondialdehyde (MDA) contents in the exudate of rat acute air pouch synovitis model induced by sc injection of 25 mg/kg carrageenan were investigated by Lowry and test tube dilution methods and thiobarbituric acid spectrophotometry Its effects on the intracellular cAMP content in and release of O ÷ 2 from Neu were further assessed by radioimmunoassay and cytochrome C reduction methods respectively The results showed that PnS 60,120,240 mg/kg ip reduced the migration of Neu and exudation of protein, lowered the content of MDA and inhibited the release of O ÷ 2 from Neu; but elevated the cAMP content of Neu in a dose dependent manner These results revealed that Pns has an obvious anti inflammatory effect and its primary action was related to the increase of cAMP content in Neu, followed by inhibition of the free radical production

重慶市中醫(yī)藥管理局課題!No:990 32