目的 研究苦豆子種子中生物堿的分離及槐果堿轉(zhuǎn)移氫化制備苦參堿的研究。方法 利用離子交換、萃取和柱層,并結(jié)合轉(zhuǎn)移氫化增大不同生物堿理化性質(zhì)差異進(jìn)行分離。結(jié)果 從苦豆子種子中分得 5種生物堿,其結(jié)構(gòu)經(jīng)元素分析、IR、MS¹ HNMR和¹³CNMR光譜鑒定為氧化苦參堿、氧化槐定堿、苦參堿、槐定堿和 lehannine。結(jié)論 首次從苦豆子種子中分得 lehm annine。

Object To study the alkaloid constituents in the seed of Sophora alopecuroides L. and isolation of lehmannine by transhydrogenation of sophocarbine to matrine present in the mixture. Methods Various processes of exchange, ion exchange and column chromatograph with very similar physical and chemical properties was successfully separated by transhydrogenation. Results 5 alkaloids were obtained and identified by elementary analysis, IR, MS, 1HNMR and 13CNMR as oxymatrine, oxysophoridine, matrine, sophoridine and lehmannine (12, 13-dehydromatrine). Conclusion Lehmannine was isolated from the seed of S. alopecuroides for the first time.

中國博士后科學(xué)基金(1999)和天津市英捷科技發(fā)展有限公司資助課題