目的 建立黃芪粉針劑的質(zhì)量控制方法。方法 采用薄層色譜 -分光光度法對黃芪粉針劑中的主要成分黃芪甲苷進行含量測定,展開劑為氯仿 -甲醇 -水 (6 5∶ 30∶ 10 ),測定波長為 418nm。結(jié)果 回歸方程 A=1.5 38C+0 .189,r=0 .9998,在 80～ 2 40μg點樣量范圍內(nèi),吸光度與點樣量呈良好的線性關(guān)系,平均回收率為 98.6 8%,RSD=2 .5 2 % (n=5 )。結(jié)論 本方法使用簡單儀器測定,結(jié)果可靠,專屬性強,可作為黃芪粉針劑的質(zhì)量控制標(biāo)準(zhǔn)

Object To develop a method for the quality control of Astrgalus membranaceus (Fisch.) Bge. powder for injection (AMPI). Methods TLC-spectrophotometry was used to determine the content of astragaloside A in AMPI. The mobile phase was chloroform-methanol-water (65∶30∶10) and detected at the wave length of 418 nm. Results The regression equation within the spotted range of 80～240 μg was A=1.538 C+ 0.189, with good linearity, r=0.999 8. The average recovery rate was 98.68% and RSD=2.52%(n=5). Conclusion The method used simple and easily available instruments and the rusults obtained were reliable and specific, suitable for the quality control of AMPI.