目的 研究微波技術(shù)對(duì)大黃游離蒽醌浸出量的影響,并與傳統(tǒng)提取方法做比較。方法 采用正交試驗(yàn)法考察微波輸出功率、藥材粒徑、浸出時(shí)間3個(gè)因素對(duì)提取效率的影響,優(yōu)選大黃游離蒽醌的最佳浸出方案。以?xún)?yōu)選出的微波浸出方案為實(shí)驗(yàn)組,以常規(guī)煎煮法及乙醇回流法為對(duì)照組,進(jìn)行平行實(shí)驗(yàn)。結(jié)果 藥材粒徑對(duì)大黃游離蒽醌浸出的影響高度顯著(P<0.01),功率對(duì)大黃游離蒽醌浸出的影響顯著(P<0.05),時(shí)間對(duì)大黃游離蒽醌的浸出有一定的影響(0.05<P<0.1)。微波浸提法對(duì)大黃游離蒽醌的提取效率明顯優(yōu)于常規(guī)煎煮法,同乙醇回流提取法相當(dāng)。結(jié)論 微波浸提法是一種提取效率高、操作簡(jiǎn)便、省時(shí)的提取中草藥活性成分的新方法,值得推廣應(yīng)用。

Object To apply microwave technique to the extraction of free anthraquinones from Rheum emodi Wall. and the methods of microwave extraction and traditional extraction were comparied. Methods The orthogonal test design was used to arrange sequent experiments to test the effect of the three main factors including microwave power, particle size of herb and extraction time on the extraction efficiency of free anthraquinones and to establish optimal extraction process. A parallel test was carried out using the three different methods including the optimal extraction process, the traditional decocting method and the alcohol refluxing method. Results Particle size of herb and microwave power are the significant factors with P<0.01 and P<0.05 respectively. Time is an affective factor on the extraction of anthraquinones(0.05<P<0.1). Microwave extraction method is superior to the traditional decocting method and equivalent to the alcohol refluxing method. Conclusion Microwave extraction method is efficient, simple and rapid for extraction of active compounds of TCM.

國(guó)家“九·五”攻關(guān)資助項(xiàng)目(編號(hào):96-C02-04-04)