目的 初步探討酸棗仁與緬棗仁的蛋白質(zhì)差異。方法 用凱氏定氮法測定蛋白質(zhì)的含量;用酸水解法測定蛋白質(zhì)的氨基酸組成;用十二烷基硫酸鈉-聚丙烯酰胺凝膠電泳(SDS-PAGE)和毛細管區(qū)帶電泳(CZE)分析蛋白質(zhì)的組分。結(jié)果 酸棗仁與緬棗仁的蛋白質(zhì)含量分別為36.13%和41.58%;除緬棗仁蛋白質(zhì)的纈氨酸和蛋氨酸明顯高于酸棗仁外,蛋白質(zhì)的其他氨基酸的組成差別不明顯;SDS-PAGE 圖譜明顯不同的是,酸棗仁有相對分子質(zhì)量為39800的蛋白質(zhì),緬棗仁為50100的蛋白質(zhì);CZE 圖譜的區(qū)別是,酸棗仁為3.1min 的峰,緬棗仁含有一個遷移時間為4.8min 的峰。結(jié)論 緬棗仁的蛋白質(zhì)含量較酸棗仁略高,且纈氨酸和蛋氨酸的含量明顯高于酸棗仁。兩種棗仁的SDS-PAGE 和CZE 圖譜均有明顯的差異存在。

Object To preliminarily study the difference of proteins in the seed of spine date and the seed of Indian jujube. Methods Protein contents were measured with Kieldahl method. Amino acids constituting the protein were determined by means of acidolysis. The proteins were analyzed using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and capillary zone electrophoresis (CZE). Results Contents of proteins in the seed of spine date and the seed of Indian jujube were 36.13% and 41.58%, respectively. The content of valine and methionine in the seed of Indian jujube were significantly more than that in the seed of spine date remarkably, and the contents of other amino acids from the seed of spine date and seed of Indian jujube were similar. Their SDS-PAGE spectra showed that the seed of spine date contained a protein with molecular weight 39800 and the seed of Indian jujube contained a protein with molecular weight 50100. In CZE spectra for the seed of spine date, there was a peak at the mobile time of 3.1min and in CZE spectra from the seed of Indian jujube, there was a peak at the mobile time of 4.8min. Conclusion Protein content in the seed of Indian jujube is more than that in the seed of spine date; The content of valine and methionine in the seed of Indian jujube is more than that in the seed of spine date remarkably. The spectra of SDS-PAGE and CZE for the seed of spine date are different from that for the seed of Indian jujube clearly.