目的 解決南方紅豆杉生長緩慢的問題。方法 考察紅豆杉細(xì)胞懸浮培養(yǎng)過程中磷的消耗,并研究了碳、氮、磷對(duì)細(xì)胞生長及細(xì)胞活性的影響。結(jié)果 碳源在懸浮培養(yǎng)中期已經(jīng)消耗殆盡,后期碳源的缺乏限制了細(xì)胞的生長。結(jié)論 補(bǔ)加碳源組與對(duì)照組相比,細(xì)胞生長率和細(xì)胞密度明顯提高,其中細(xì)胞生長率約提高了83%。

Object To solve the problem that Taxus chinensis var. mairei (Lemee et Lévl.) Cheng et L. K.Fu grows slowly. Methods The consumption of phosphorus during the cell suspension culture and the effect of fed-batch carbohydrate, nitrogen and phosphorus on cell growth and living-cell activity were assayed. Results The carbohydrate was exhausted in the middle phase of suspension culture, dificiency of carbohydrate led to inhibition of cell growth in the late culture. Conclusion Compared with the control group, the cell growth rate and the cell density in the fed-batch carbohydrate group were increased significantly, and the cell growth rate was up to 83%.

天津市重點(diǎn)攻關(guān)項(xiàng)目(963107211);國家教委跨世紀(jì)優(yōu)秀人才培養(yǎng)計(jì)劃基金資助