目的 體外觀察164種中藥95%乙醇提取物對白色念珠菌、啤酒酵母和威克海姆原藻3種真菌的作用,并對其中抑菌效果明顯的中藥中的主要成分進行抗真菌藥敏試驗。方法 半固體藥基混合法,微量稀釋法。結(jié)果 22種中藥乙醇提取物對1種或幾種真菌顯示較強的抑制作用,所試驗的單體化合物對3種真菌無顯著抑制作用。結(jié)論 首次對中藥95%乙醇提取物應(yīng)用半固體藥基混合法進行抗真菌活性篩選,土槿皮、牡丹皮95%乙醇粗提物對3種真菌均有較強的抑制作用。

Object To screen antifungal agents against Candida albicans (Robin) Berkh, Saccharomyces cerevisiac Hansen GL-7 and Prototheca wickerhamii from 164 traditional Chinese medicines (TCMs). Methods The extracts of 164 TCMs were prepared with 95% ethanol and the antifungal activity of these extracts against above three fungi was measured by semisolid agar dilution method. Results The ethanol extracts from 22 TCMs exhibited potent antifungal activity. Conclusion The antifunal activity of the ethanol extracts from TCMs was measured by using semisolid agar dilution method for the first time. The ethanol extracts from the bark of Pseudolarix kaempferi Gord. and Paeonia suffruticosa Andr. are more potent antifungal agents than those of tested other medicines.

國家自然科學基金(N0.30070917)