目的研究補陽還五湯的抗血栓有效部位及其活性成分.方法用多種色譜、核磁共振波譜和單晶X-衍射方法對補陽還五湯水煎液的生物堿類化合物進行了系統(tǒng)的分離和結(jié)構(gòu)鑒定.結(jié)果分離并鑒定了4個生物堿類化合物,分別為N-(3'-馬來酰亞胺)-5-羥甲基-2-吡咯甲醛(Ⅰ)、4-氨甲?；?2-吡咯甲酸(Ⅱ), N-(1'-D-去氧木糖醇基)-6,7-二甲基-1,4-二氫-2,3-喹喔啉二酮(Ⅲ)及2,3,4,9-四氫-1H-吡啶駢[3,4-b]吲哚-3-羧酸(Ⅳ).結(jié)論以上化合物均首次從補陽還五湯中分離得到且化合物Ⅰ~Ⅲ為新化合物.

Objective To study the antithrombotic compounds and bioactive fraction from Buyang Huanwu Decoction(BHD).Methods Four alkalo-ids have been isolated from BHD by repeated chromatographies.Their structures were elucidated by means of spectral and single-crystal X-ray diffraction analysis.Results They are N-(3'-maleimidyl)-5-hydroxymethyl-2-pyrrole formaldehyde(I),4-car-bamoyl-2-pyrrole carboxylic acid(Ⅱ),N-(1 7-D-deoxyxylit01y1)-6,7-dimethyl-1,4-dihydro-2,3-quinoxalinedione(Ⅲ),and 2,3,4,9-tetra-hydro-1H-pyrido E3,4-b3 indole-3-earboxylic acid(IV).Conclusion All these compounds are isolated from BHD for the first time and compounds I-Ⅲare new alkaloids.

國家"九五"攀登項目(970211018)