目的研究高效液相色譜制備-枝蒿酮酸對(duì)照品的方法.方法原料用95%乙醇提取,濃縮成浸膏后用醋酸乙酯萃取.醋酸乙酯部位用100~200目硅膠柱色譜分離,用石油醚-醋酸乙酯(3:1)梯度洗脫,收集樣品進(jìn)行HPLC分離制備.結(jié)果該法所得產(chǎn)品用HPLC歸-化法定量,質(zhì)量分?jǐn)?shù)大于98%.結(jié)論本方法簡(jiǎn)便,產(chǎn)品質(zhì)量高,-枝蒿酮酸產(chǎn)品可用做分析方法的對(duì)照品.

Objective To establish a preparative method for rupestonic acid reference substance fromArtemisia rupestrisby HPLC. Methods After raw material being extracted with 95%ethanol,the ethanol extracts were evaporated to dryness under reduced press-ure.Then the dry powder was extracted by ethyl acetate,the ethyl acetate extract was fractionated by column chromatography on silica gel(100-200mesh)using petroleumbenzine-ethyl acetate(3:1)as the eluent.The collected fraction was purified by preparative HPLC. Results The purity of the product quantitated by normality was over 98%.Conclusion The developed method iS simple and rapid and the product of rupestonic acid can be used as reference substance.