目的探討石菖蒲及其成分α-細(xì)辛醚對戊四氮(PTZ)誘發(fā)的實(shí)驗(yàn)性癲癇幼鼠運(yùn)動(dòng)行為和空間學(xué)習(xí)記憶能力的影響.方法W istar幼鼠隨機(jī)分為正常對照組(NC)和致癇模型組(A)、苯巴比妥鈉組(B)、石菖蒲組(C)、α-細(xì)辛醚組(D),以ip PTZ 60mg/kg建立癲癇模型,各組ig給藥.動(dòng)態(tài)觀察各組幼鼠在斜板試驗(yàn)、懸吊試驗(yàn)、自主活動(dòng)試驗(yàn)中行為運(yùn)動(dòng)的變化以及在Morris水迷宮試驗(yàn)中空間學(xué)習(xí)記憶能力的改變.結(jié)果A組動(dòng)物的轉(zhuǎn)體能力、懸吊能力、自主活動(dòng)能力和空間學(xué)習(xí)記憶能力明顯下降,與驚厥發(fā)作嚴(yán)重程度呈正相關(guān),B組次之,與其他各組比較差異均顯著(P<0.05).而C組、D組各項(xiàng)結(jié)果與正常對照組比較差異不顯著.結(jié)論PTZ誘發(fā)的幼鼠多次驚厥發(fā)作損害其行為運(yùn)動(dòng)能力和空間學(xué)習(xí)記憶能力,與苯巴比妥鈉相比,α-細(xì)辛醚和石菖蒲能夠有效地逆轉(zhuǎn)PTZ所致的損害.

Objective To explore the effect ofAcorus gramineusand its component α-asarone on the ability of the experimental you-ng rats with epilepsy induced by pentylenetetrazol(PTZ)to move and memorize.Methods Wistar young rats were randomly divided into normal control group(NC),epileptic model group(A),phenobarbital sodium group(B),A.gramineusgroup(C),and α-asarone group (D).The epileptic model was established by ip PTZ 60 mg/kg and rats in all groups were given the tested drugs by ig.Changes of move-ment in inclined plate test,over hanging test,autonomic movement test and changes of spatial learning ability and memory in Morris water maze test were observed in Wistar young rats.Results The ability of rats in groupAto writhe body,suspend body,and move automatically as well as spatial learning ability and memory were obviously decreased,which had positive correlation to the extent of eclampsia.Group B is secondary to group A.There were significant differences between the above mentioned results and the other three groups(P<0.05).But there was no obvious difference among group NC,group C,and group D.Conclusion The damage of moving and learning ability and memory function of the young rats caused by frequent epilepsy paroxysm induced by PTZ could be reve-rsed more efficiently by α-asarone and A.gramineus than phenobarbital sodium.

吉林省科委自然科學(xué)基金資助項(xiàng)目(20030430);吉林省中醫(yī)藥管理局資助項(xiàng)目(2004-079)