目的在蘭科石斛屬藥用植物鑒定中應(yīng)用新的分子標(biāo)記.方法擴(kuò)增并測(cè)定9種石斛屬植物線粒體中NADH脫氫酶亞基1編碼基因(nad 1)內(nèi)含子2(intron 2)的全長序列.結(jié)果比對(duì)后的nad 1 intron 2序列長872bp,其中有17個(gè)變異位點(diǎn),可以鑒別除粉花石斛Dendrobium lodd igesii以外的8種植物.結(jié)論線粒體nad 1 intron2序列可以作為-種新的分子標(biāo)記用于石斛屬植物的鑒定.

Objective Application of a new molecular marker to the identification ofDendrobium(Orchidaceae)species.Methods Complete sequences of the mitochondrial nad 1 intron 2 for nine species ofDendrobium Sw.were amplified and determined. Results Seventeen variable sites were found in the aligned 872 bp of nad 1 intron 2 sequences.Eight of the nine Dendrobium species except D.loddigesiicould be identified by the nad 1 intron 2 sequences.Conclusion The mitochondrial nad 1 intron 2 sequences could be used as a new molecular marker for the identification ofDendrobium species.

國家自然科學(xué)基金資助項(xiàng)目(30171144)