-6~5.83×10-5;韌皮部為2.3×10-6 ~1.030×10 -4.結(jié)論雷公藤和昆明山海棠不同個(gè)體雷公藤甲素質(zhì)量分?jǐn)?shù)最高值與最低值相差約50倍,不同居群雷公藤甲素質(zhì)量分?jǐn)?shù)最高值與最低值相差10多倍,不同來(lái)源的藥材質(zhì)量差異極大,嚴(yán)重影響用藥的安全性;雷公藤甲素質(zhì)量分?jǐn)?shù)高的居群位于浙江西南部和中部、湖南新寧、貴州雷山和安徽黃山;湖南新寧、貴州雷山和浙江江山居群中有雷公藤甲素質(zhì)量分?jǐn)?shù)極高的個(gè)體,值得進(jìn)-步研究,以尋找質(zhì)量分?jǐn)?shù)高的優(yōu)良單株.;Objective To determine the triptolide in different populations and individuals of three species ofTripterygiumHook.f. (Tripterygium wilfordii,T.hypoglaucum,andT.regellii),establish a foundation for evaluating the quality of species inTripterygiumHook.f.,and find good germplasm ofTripterygium Hook.f.Methods HPLC method for determining triptolide was established.The triptolide in xylem and phloem of 25 populations and 91 individuals from main distribution areas in China was determined.Results The triptolide inT.regelliiwas very low.The interspecies difference betweenT.wilfordii and T.hypoglaucum was not conspicuous.The range of triptolide of the individual was 1.0×10-6-5.83×10-5in xylem and 2.3×10-6-1.030×10-4 in phloem.Conclusion The quality of species inTripterygium Hook.f.from different resources varies significantly.It will affect seriously the security of administering drugs.The highest value of triptolide is about 50 times as the lowest value in different individuals and more than 1 0 times in different populations. The populations with higher triptolide are in the southwest and middle parts of Zhejiang Province,Xinning,Hunan Province,Leishan, Guizhou Province,and Huangshan,Anhui Province.The individuals with very high contents of triptolide exists in Xinning,Hunan Provin-ce,Leishan,Guizhou Province,and Jiangshan,Zhejiang Province populations.So further study on the three populations should be carried out to find good individuals."/>

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雷公藤屬3種植物不同群體和個(gè)體中雷公藤甲素的研究

Triptolide in different populations and individuals of three species ofTripterygium Hook.f.

發(fā)布日期：2015-01-07
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    [關(guān)鍵詞]
    [摘要]
    目的測(cè)定雷公藤屬3種植物(雷公藤、昆明山海棠和黑蔓)不同群體和個(gè)體中雷公藤甲素(triptolide),為評(píng)價(jià)雷公藤藥材質(zhì)量和尋找雷公藤的優(yōu)質(zhì)種質(zhì)奠定基礎(chǔ).方法建立雷公藤甲素HPLC測(cè)定方法,并測(cè)定了全國(guó)主要分布區(qū)25個(gè)群體91個(gè)個(gè)體的木質(zhì)部和韌皮部中的雷公藤甲素.結(jié)果黑蔓的雷公藤甲素質(zhì)量分?jǐn)?shù)很低;雷公藤和昆明山海棠種間的雷公藤甲素差異不明顯,個(gè)體質(zhì)量分?jǐn)?shù)木質(zhì)部為1.0×10-6~5.83×10-5;韌皮部為2.3×10-6 ~1.030×10 -4.結(jié)論雷公藤和昆明山海棠不同個(gè)體雷公藤甲素質(zhì)量分?jǐn)?shù)最高值與最低值相差約50倍,不同居群雷公藤甲素質(zhì)量分?jǐn)?shù)最高值與最低值相差10多倍,不同來(lái)源的藥材質(zhì)量差異極大,嚴(yán)重影響用藥的安全性;雷公藤甲素質(zhì)量分?jǐn)?shù)高的居群位于浙江西南部和中部、湖南新寧、貴州雷山和安徽黃山;湖南新寧、貴州雷山和浙江江山居群中有雷公藤甲素質(zhì)量分?jǐn)?shù)極高的個(gè)體,值得進(jìn)-步研究,以尋找質(zhì)量分?jǐn)?shù)高的優(yōu)良單株.
    [Key word]
    [Abstract]
    Objective To determine the triptolide in different populations and individuals of three species ofTripterygiumHook.f. (Tripterygium wilfordii,T.hypoglaucum,andT.regellii),establish a foundation for evaluating the quality of species inTripterygiumHook.f.,and find good germplasm ofTripterygium Hook.f.Methods HPLC method for determining triptolide was established.The triptolide in xylem and phloem of 25 populations and 91 individuals from main distribution areas in China was determined.Results The triptolide inT.regelliiwas very low.The interspecies difference betweenT.wilfordii and T.hypoglaucum was not conspicuous.The range of triptolide of the individual was 1.0×10-6-5.83×10-5in xylem and 2.3×10-6-1.030×10-4 in phloem.Conclusion The quality of species inTripterygium Hook.f.from different resources varies significantly.It will affect seriously the security of administering drugs.The highest value of triptolide is about 50 times as the lowest value in different individuals and more than 1 0 times in different populations. The populations with higher triptolide are in the southwest and middle parts of Zhejiang Province,Xinning,Hunan Province,Leishan, Guizhou Province,and Huangshan,Anhui Province.The individuals with very high contents of triptolide exists in Xinning,Hunan Provin-ce,Leishan,Guizhou Province,and Jiangshan,Zhejiang Province populations.So further study on the three populations should be carried out to find good individuals.
    [中圖分類(lèi)號(hào)]
    [基金項(xiàng)目]
    浙江省林業(yè)重大科研項(xiàng)目"木本藥材高效栽培技術(shù)及有效成分提取工藝的研究"資助項(xiàng)目(2002A01)
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