2O2脫色素、Sephadex G-200色譜柱分離純化得到均一的紅芪多糖HPS4-1A。以GC法、高效液相凝膠色譜(HPGC)法、凝膠滲透色譜-多角度激光散射儀聯(lián)用(GPC-MALLS)法、元素分析、苯酚硫酸法、Bradford法研究其理化性質(zhì);以GPC-MALLS法對(duì)其構(gòu)象進(jìn)行初步分析。采用甲基化、部分酸水解、以及NMR研究其連接方式、主鏈和支鏈結(jié)構(gòu)及分支點(diǎn)狀況。結(jié)果 HPS4-1A的絕對(duì)分子質(zhì)量為7.386×104,相對(duì)分子質(zhì)量6.68×105以上;由鼠李糖、阿拉伯糖、葡萄糖、半乳糖組成,其摩爾比為1∶2∶1∶2,主鏈骨架由1, 5、1, 3, 5連接的α-L-呋喃阿拉伯糖和1, 6、1, 2, 6連接的α-D-吡喃半乳糖組成,側(cè)鏈分支點(diǎn)位于阿拉伯糖的3位與半乳糖的2位。側(cè)鏈分支由1, 4、1, 4, 6-α-D-吡喃葡萄糖,1, 2、1, 2, 4-α-L-呋喃鼠李糖組成。主鏈末端連接主要是阿拉伯糖,并且連接在1, 5連接的阿拉伯糖的5位。側(cè)鏈末端連接主要是葡萄糖,還有少量的阿拉伯糖。結(jié)論 HPS4-1A為一種新的中性紅芪雜多糖,其構(gòu)象為無(wú)規(guī)線(xiàn)團(tuán)狀,并且相對(duì)分子質(zhì)量分布呈單分散性。;Abstract: Objective To study the chemical structural features and conformation of polysaccharide HPS4-1A from Hedysarum polybotrys. Methods A fraction of water-soluble neutral polysaccharide was obtained from H. polybotrys by aqueous extraction followed by precipitation with ethanol. The homogeneous polysaccharide named HPS4-1A was obtained after treated with Savage method and H2O2, and purified with Sephadex G-200 gel filtration chromatography. Then GC, HPGC, GPC-MALLS, element alanalyser, phenol sulfuric acid method, and Bradford method were used to study the physicochemical property of HPS4-1A; The conformation was primarily analyzed with GPC-MALLS method. The connection, main chain and branched-chain structures, and the condition of branching point were studied by methylation, partial acid hydrolysis, and NMR method. Results The absolute and relative molecular weights of HPS4-1A were 7.386 × 104 and above 6.68 × 105; HPS4-1A was a heteropolysaccharide and consisted of L-rhamnose, L-arabinose, D-glucose, and D-galactose (1︰2︰1︰2). HPS4-1A proved to be a neutral sugar, with 1, 6- and 1, 2, 6-α-D-galactopyranosyl and 1, 5- and 1, 3, 5-α-L-arabinofuranosyl residues in backbone, and 1, 4- and 1, 4, 6-α-D-glucopyranosyl and 1, 2- and 1, 2, 4-α-L-rhamnofuranosyl residues in branches. Arabinose mainly connected the end of backbone, and glucose and a small quantity of arabinose mainly connected the end of branches. Conclusion HPS4-1A is a new neutral heteropolysaccharide from H. polybotrys. HPS4-1A has a random coil state conformation with monodisperse mass distribution."/>