目的 研究肺筋草Aletris spicata全草的化學成分。方法 利用正、反相硅膠柱色譜、薄層色譜、Sephadex LH-20柱色譜及半制備高效液相色譜等方法進行分離純化，然后根據(jù)理化性質(zhì)和波譜數(shù)據(jù)鑒定化合物的結構，并對其中的三萜類化合物（化合物1～3）進行了抗腫瘤活和抗菌活性初步篩選。結果 從肺筋草的干燥全草中分離得到了9個化合物，分別鑒定為24-甲基-9, 19-環(huán)羊毛甾-24-烯-3-醇（1）、24-甲基-9, 19-環(huán)羊毛甾-25-烯-3-醇（2）、24, 24-二甲基-環(huán)木菠蘿烷-3-醇（3）、美商陸酚A（4）、異美商陸酚A（5）、9′-methyl americanol A（6）、1-(4′-羥基苯基)-7-(3″-甲氧基-4″-羥基苯基)-4-烯-3-庚酮（7）、methyl-9, 12, 13-trihydroxyoctadeca-10E, 15Z-dienoate（8）、5-羥甲基-2-呋喃甲醛（9）。結論 所有化合物均為首次從該屬植物中分離得到?；衔?b>1～3無抗腫瘤和抗菌活性。

Objective To study the chemical constituents of Aletris spicata. Methods The compounds were isolated and repeatedly purified by silica gel, TLC, and Sephadex LH-20 column chromatography as well as semi-Prep HPLC, and their structures were elucidated by means of spectral analysis and physicochemical properties. The antitumor and antibacterial activities of triterpenoids (compounds 1—3) were primarily screened. Results Nine compounds were obtained and identified as 24-methyl-9, 19-cyclolanost- 24-en-3-ol (1), 24-methyl-9, 19-cyclolanost-25-en-3-ol (2), cycloneolitsol (3), americanol A (4), isoamericanol A (5), 9′-methyl americanol A (6), 1-(4′-hydroxyphenyl)-7-(3″-methoxyl-4″-hydroxyphenyl)-heptene-4-en-3-one (7), methyl-9, 12, 13-trihydroxy- octadeca-10E, 15Z-dienoate (8), and 5-hydroxymethyl-2-furancarboxaldehyde (9). Conclusion All the compounds are isolated from the plants in Aletris L. for the first time. No anti-tumor and anti-bacterial activities are found in compounds 1—3.

國家自然基金項目（81160390）；貴州省優(yōu)秀青年科技人才培養(yǎng)計劃項目（黔科合人字 [2011] 33號）；貴州省優(yōu)秀科技教育人才省長專項資金項目（黔省專合字 [2010] 71號）