目的 研究甘草次酸（GA）-丹參酮II_A（Tan II_A）復方脂質體（GT-Lip）的制備工藝。方法 以大豆卵磷脂（SPC）和膽固醇（Ch）為膜材，薄膜分散探頭超聲法制備GT-Lip。通過單因素考察確定水合溫度和探頭超聲功率，正交設計法優(yōu)化處方工藝；低速離心法測定脂質體中GA與Tan II_A包封率，動態(tài)光散射粒徑儀測定脂質體粒徑與Zeta電位，透射電鏡測定脂質體形態(tài)。結果 優(yōu)化處方工藝為SPC-Ch質量比6∶1，SPC-Tan II_A物質的量之比30∶1，SPC-GA物質的量之比24∶1，水合溫度為30 ℃，探頭超聲條件為380 W超聲5 min；制備得到的GT-Lip中Tan II_A、GA的包封率分別為（81.50±0.76）%、（98.63±0.90）%（n＝3），平均Zeta電位為（?19.00±0.98）mV（n＝3），平均粒徑為（120.5±1.62）nm（n＝3）。結論 優(yōu)化的GT-Lip制備工藝穩(wěn)定可行。

Objective To study the preparation technology of glycyrrhetinic acid (GA)-tanshinone II_A (Tan II_A) compound liposomes (GT-Lip). Methods The compound liposomes were made of soybean phosphatidylcholine (SPC) and cholesterol (Ch) by film dispersion ultrasonic probe method. Hydration temperature and ultrasonic power were optimized by single factor tests and the optimum formulation was selected by orthogonal design. The encapsulation efficiencies (EE) of GA and Tan II_A were determined by low-speed centrifugation. The particle size and Zeta potential of liposomes were detected by dynamic light scattering particle size meter. Transmission electron microscopy was used to observe morphous. Results The optimal preparation conditions were as follows weight ratio of SPC-Ch 6∶1, molar ratio of SPC-Tan II_A and SPC-GA was 30∶1 and 24∶1, hydration temperature 30 ℃, and ultrasonic power 380 W for 5 min. Using the optimal method, the EE values of GA and Tan II_A in GT-Lip were (81.50 ± 0.76)% and (98.63 ± 0.90)% (n = 3), and the average particle size of liposomes was (120.5 ± 1.62) nm and the Zeta potential of liposomes was (?19.00 ± 0.98) mV (n = 3). Conclusion The optimal preparation method of GT-Lip in this study is stable and feasible.

國家自然科學基金資助課題（81202928）；北京市自然科學基金資助課題（7132118）；北京中醫(yī)藥大學復方中藥制藥研究創(chuàng)新團隊項目（2011-CXTD-13）