A(Tan IIA)復(fù)方脂質(zhì)體(GT-Lip)的制備工藝。方法 以大豆卵磷脂(SPC)和膽固醇(Ch)為膜材,薄膜分散探頭超聲法制備GT-Lip。通過單因素考察確定水合溫度和探頭超聲功率,正交設(shè)計(jì)法優(yōu)化處方工藝;低速離心法測(cè)定脂質(zhì)體中GA與Tan IIA包封率,動(dòng)態(tài)光散射粒徑儀測(cè)定脂質(zhì)體粒徑與Zeta電位,透射電鏡測(cè)定脂質(zhì)體形態(tài)。結(jié)果 優(yōu)化處方工藝為SPC-Ch質(zhì)量比6∶1,SPC-Tan IIA物質(zhì)的量之比30∶1,SPC-GA物質(zhì)的量之比24∶1,水合溫度為30 ℃,探頭超聲條件為380 W超聲5 min;制備得到的GT-Lip中Tan IIA、GA的包封率分別為(81.50±0.76)%、(98.63±0.90)%(n=3),平均Zeta電位為(?19.00±0.98)mV(n=3),平均粒徑為(120.5±1.62)nm(n=3)。結(jié)論 優(yōu)化的GT-Lip制備工藝穩(wěn)定可行。;Objective To study the preparation technology of glycyrrhetinic acid (GA)-tanshinone IIA (Tan IIA) compound liposomes (GT-Lip). Methods The compound liposomes were made of soybean phosphatidylcholine (SPC) and cholesterol (Ch) by film dispersion ultrasonic probe method. Hydration temperature and ultrasonic power were optimized by single factor tests and the optimum formulation was selected by orthogonal design. The encapsulation efficiencies (EE) of GA and Tan IIA were determined by low-speed centrifugation. The particle size and Zeta potential of liposomes were detected by dynamic light scattering particle size meter. Transmission electron microscopy was used to observe morphous. Results The optimal preparation conditions were as follows weight ratio of SPC-Ch 6∶1, molar ratio of SPC-Tan IIA and SPC-GA was 30∶1 and 24∶1, hydration temperature 30 ℃, and ultrasonic power 380 W for 5 min. Using the optimal method, the EE values of GA and Tan IIA in GT-Lip were (81.50 ± 0.76)% and (98.63 ± 0.90)% (n = 3), and the average particle size of liposomes was (120.5 ± 1.62) nm and the Zeta potential of liposomes was (?19.00 ± 0.98) mV (n = 3). Conclusion The optimal preparation method of GT-Lip in this study is stable and feasible."/> A;復(fù)方脂質(zhì)體;薄膜分散探頭超聲法;低速離心法;glycyrrhetinic acid; tanshinone IIA; compound liposomes; film dispersion ultrasonic probe method; low-speed centrifugation"/>

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首頁 > 過刊瀏覽>2013年第44卷第7期 >2013,44(7):816-819. DOI:10.7501/j.issn.0253-2670.2013.07.008
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甘草次酸-丹參酮IIA復(fù)方脂質(zhì)體處方優(yōu)化及制備工藝研究

Formula optimization and preparation technology of glycyrrhetinic acid- tanshinone IIA compound liposomes

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