18柱(250 mm×4.6 mm,5 μm),在230 nm波長下,以乙腈-0.05%磷酸水溶液進(jìn)行梯度洗脫,測(cè)定了44批強(qiáng)肝膠囊樣品;應(yīng)用相似度分析軟件建立強(qiáng)肝膠囊指紋圖譜的共有模式,并對(duì)色譜峰進(jìn)行了指認(rèn)。結(jié)果 建立強(qiáng)肝膠囊HPLC指紋圖譜,在確定的方法下,得到44批強(qiáng)肝膠囊的色譜圖,并獲得了44批樣品的相似度;確定共有峰14個(gè),其中13個(gè)歸屬到各藥材,11個(gè)已確認(rèn)成分。結(jié)論 強(qiáng)肝膠囊的HPLC指紋圖譜的構(gòu)建和共有色譜峰成分的確定為強(qiáng)肝膠囊藥品質(zhì)量控制提供參考。;Objective To establish HPLC fingerprint for Qianggan Capsule and identify the chemical pattern recognition. Methods The HPLC method was used with Diamonsil-C18 column (250 mm × 4.6 mm, 5 μm), under the 230 nm detection wavelength, gradient eluted by acetonitrile-0.05% H3PO4. The common mode of HPLC fingerprint for 44 batches of Qianggan Capsule was established with similarity analyses and the peaks were identified. Results Under the selected spectrum condition, the HPLC fingerprint for 44 batches of Qianggan Capsule and similarities for 44 batches of samples were obtained. Fourteen peaks were found, 13 belong to herbs and 11 were assigned. Conclusion The establishment of HPLC fingerprint of Qianggan Capsule and the identification of peaks provide more reference for the quality control of Qianggan Capsule."/>