目的 研究桃仁提取物對寒凝血瘀證（寒證）和瘀熱互結(jié)證（熱證）大鼠血液循環(huán)障礙的不同藥效。方法 80只大鼠隨機(jī)均分為寒證對照組、寒證模型組、桃仁提取物（8 g/kg）組、川芎（8 g/kg）陽性對照組，以及熱證對照組、熱證模型組、桃仁提取物（8 g/kg）組、丹參（14 g/kg）陽性對照組。分別采用全身低溫冷凍和注射角叉菜膠法建立寒證、熱證大鼠模型，造模同時各給藥組每天ig給藥1次，連續(xù)給藥7 d。檢測微循環(huán)血流速率、全血黏度；取大鼠心、肺、肝、腎、脾進(jìn)行組織病理學(xué)觀察，病理圖像分析軟件測量微小血管管徑，觀察血栓、組織損傷等情況。結(jié)果 與模型組相比，桃仁提取物能明顯加快寒證和熱證大鼠的血流速率（P＜0.05），降低全血黏度（P＜0.05），使寒證大鼠小動脈收縮、熱證大鼠小動脈舒張（P＜0.05）；根據(jù)器官損傷嚴(yán)重度評分得知桃仁提取物可減輕寒證大鼠心、肺、肝、腎器官的損傷（P＜0.05），但僅能減輕熱證大鼠的腎損傷（P＜0.05）。結(jié)論 桃仁提取物能改善寒凝血瘀證和瘀熱互結(jié)證大鼠的血液循環(huán)障礙；對不同證型大鼠小動脈的舒縮狀態(tài)和心、肺、肝、腎的保護(hù)作用不同。

Objective To study the different pharmacodynamic effects of Persicae Semen extract (PSE) on the blood circulation disorder of rats induced by cold congeal and blood stasis syndrome (cold syndrome, CS), and heat congeal and blood stasis syndrome (heat syndrome, HS). Methods Eighty rats were randomly divided into eight groups including normal control group of CS, model group of CS, PSE (8 g/kg) group of CS, Chanxiong Rhizoma (8 g/kg) group of CS, normal control group of HS, model group of HS, PSE (8 g/kg) group of HS, and Salviae Miltiorrhizae Radix (14 g/kg) group of HS. The two rat models were established by freezing and injection of carrageenan solution. The rats in each group were ig administered once daily for 7 d. The blood flow velocity (Fve) and blood viscosity (Vis) were detected; The organs (such as heart, lung, liver, kidney, and spleen) were taken and observed by histopathological analysis; The diameters of tiny blood vessels were measured by pathology image analysis software, and the thrombosis and tissue injury were observed. Results PSE increased Fve but decreased Vis in CS and HS rats (P < 0.05), made the small artery in CS rats systolic, while that in HS rats diastolic (P < 0.05). The organ injury severity score indicated PSE could attenuate the injury of many organs (such as heart, lung, liver, and kidney) in CS rats,but only attenuated the injury of kidney in HS rats (P < 0.05). Conclusion PSE could improve the blood circulation disorder induced by CS and HS. The different effects of PSE on the two kinds of syndromes include the regulation of the diastolic and systolic states of rat small artery and the different protection of rat organs.

國家科技部“973”計劃課題（2007CB512602）；廣西科學(xué)基金項目（桂科基09320005）