[關鍵詞]
[摘要]
目的 研究人工發(fā)酵的斜生褐孔菌菌質(zhì)對鏈脲佐菌素(STZ)致2型糖尿病大鼠的影響,并與野生斜生褐孔菌菌核進行比較。方法 高糖、高脂飼料飼養(yǎng)SD大鼠6周,單次ip給予STZ 35 mg/kg制備2型糖尿病模型。將模型大鼠隨機分成模型組、斜生褐孔菌菌質(zhì)(菌質(zhì))1 g/kg組、野生斜生褐孔菌菌核(菌核)1 g/kg組和鹽酸二甲雙胍(0.2 g/kg)陽性對照組。連續(xù)ig給藥10周后,檢測各組大鼠體質(zhì)量以及空腹血糖、胰島素、糖化血紅蛋白(HbA1c)、血清游離脂肪酸(FFA)、總膽固醇(TC)、三酰甘油(TG)、高密度脂蛋白-膽固醇(HDL-C)、低密度脂蛋白-膽固醇(LDL-C)、超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽過氧化物酶(GSH-Px)等水平,并進行口服葡萄糖耐量實驗(OGTT)。結果 給藥10周后,與模型組比較,菌質(zhì)組和菌核組大鼠的體質(zhì)量明顯增加(P<0.05),血糖明顯降低(P<0.05),血清FFA、TC、TG和MDA水平下降(P<0.05),SOD、GSH-Px水平升高(P<0.05);菌質(zhì)組與菌核組的上述指標無顯著差異。結論 斜生褐孔菌菌質(zhì)能緩解STZ致2型糖尿病大鼠癥狀,具有降低血糖、調(diào)節(jié)血脂、改善氧化應激的作用,且作用強度與野生斜生褐孔菌菌核相當。
[Key word]
[Abstract]
Objective To compare the effects of fungal substance and sclerotium in Inonotus obliquus on the expeimental rats with type 2 diabetes mellitus induced by Streptozotocin (STZ). Methods The type 2 diabetic rat model was established by ip injection of STZ (35 mg/kg) on SD rats after the rats were fed with high sugar and high fat for 6 weeks. Then, the rats were divided into model, fungal substance (I. obliquus fungal substance 1 g/kg), sclerotium (I. obliquus sclerotium 1 g/kg), and positive control (metformin hydrochloride 0.2 g/kg) groups. After 10 weeks of administration, the body weight, fasting blood glucose (FBG), insulin (INS), glycated metabolism (HbAlc), free fatty acid (FFA), cholesterin (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GSH-Px), and other blood biochemical indexes were measured, and the oral glucose tolerance test (OGTT) was carried out. Results After 10 weeks of administration, compared with the model group, the body weight and the SOD and GSH-Px levels of diabetic rats were significantly increased in fungal substance and sclerotium groups (P < 0.05), and the blood glucose and FBG, FFA, TC, TG, and MDA levels in the serum of diabetic rats were significantly decreased (P < 0.05). The effects between fungal substance and sclerotium groups were statistically nonsignificant on the above indexes. Conclusion The fungal substance of I. obliquus could relieve the symptom, lower the blood glucose, regulate the blood lipid, and improve the oxidative stress of experimental rats with type 2 diabetes mellitus, and the effects are similar with sclerotium group of wild I. obliquus.
[中圖分類號]
[基金項目]
江西省節(jié)能減排技術示范工程項目(2009AE00030);江西省教育廳科學技術研究項目(GJJ10550)