目的 建立牡丹皮的HPLC指紋圖譜。方法 色譜柱為Dikma Diamonsil C₁₈色譜柱（250 mm×4.6 mm，5 μm）；以乙腈-水（含0.085%磷酸）為流動(dòng)相梯度洗脫；柱溫35 ℃；體積流量1 mL/min；檢測(cè)波長(zhǎng)254 nm（0～21.5 min），230 nm（21.5～45 min），254 nm（45～60 min）；進(jìn)樣量20 μL。采用高分辨LC-MS/MS技術(shù)進(jìn)行色譜峰指認(rèn)。結(jié)果 該方法精密度、穩(wěn)定性和重復(fù)性良好。采用高分辨LC-MS/MS方法對(duì)20個(gè)共有峰中的18個(gè)色譜峰進(jìn)行了定性鑒別。采用該方法測(cè)定了10批不同產(chǎn)地的牡丹皮，其相似度均在0.96以上。結(jié)論 本實(shí)驗(yàn)為牡丹皮的全面質(zhì)量評(píng)價(jià)奠定了基礎(chǔ)。

Objective To establish the fingerprint of Moutan Cortex by HPLC. Methods The chromatographic fingerprint was obtained with Dikma Diamonsil C₁₈ column (250 mm × 4.6 mm, 5 μm) and gradient eluted with acetonitrile and water (with 0.085% phosphoric acid). The column temperature was maintained at 35 ℃, and the flow rate was 1 mL/min. The detection wavelength was 254 (0—21.5 min), 230 (21.5—45 min), and 254 nm (45—60 min). The injection volume was 20 μL. The peaks were identified by LC-MS/MS. Results The method had good precision, stability, and repeatability. Eighteen chromatographic peaks among 20 common peaks were identified by LC-MS/MS. Ten batches of Moutan Cortex were determined, and the similarities were above 0.96. Conclusion The study lays the foundation for the full quality evaluation of Moutan Cortex.

國(guó)家“重大新藥創(chuàng)制” 科技重大專項(xiàng)課題（2011ZX09201-201-13，2012ZX09301003-001-004）