目的 優(yōu)化細(xì)柱五加莖多糖的閃式提取工藝，并研究其細(xì)胞毒性和免疫活性。方法 運(yùn)用球面對(duì)稱設(shè)計(jì)試驗(yàn)，綜合考慮料液比、提取溫度、提取時(shí)間3因素，優(yōu)化細(xì)柱五加莖多糖的閃式提取工藝；對(duì)閃式提取法與回流、超聲2種傳統(tǒng)提取方法進(jìn)行比較。通過(guò)體外試驗(yàn)對(duì)提取所得多糖的細(xì)胞毒性和免疫活性進(jìn)行初步研究。結(jié)果 細(xì)柱五加莖多糖閃式提取最佳工藝為料液比1∶13.2，提取溫度80 ℃，提取時(shí)間420 s。在此條件下，細(xì)柱五加莖中多糖提取率為0.78%，且優(yōu)于回流和超聲法提取所得多糖提取率。體外活性實(shí)驗(yàn)結(jié)果顯示，細(xì)柱五加莖多糖質(zhì)量濃度在10～20 μg/mL對(duì)RAW 264.7細(xì)胞無(wú)明顯毒性，在10～40 μg/mL能夠促進(jìn)RAW 264.7細(xì)胞NO的分泌。結(jié)論 建立了穩(wěn)定、簡(jiǎn)便的細(xì)柱五加莖多糖的閃式提取工藝方法, 初步研究了其多糖的免疫活性，為合理的開發(fā)細(xì)柱五加資源提供理論依據(jù)。

Objective To optimize the extraction process for polysaccharides from the stems of Acanthopanax gracilistylus by smashing tissue extraction (STE) and to further investigate their cytotoxicity and immunological activities. Methods Extraction process for the polysaccharides from the stems of A. gracilistylus was optimized by using the spherical symmetrical design test, and three factors were considered (material-liquid ratio, extraction temperature, and extraction time). The test compared STE with two traditional extraction methods, reflux extraction and ultrasonic extraction. The bioassay tests of cytotoxicity and immunological activities on polysaccharides extracted were studied in vitro. Results The optimal extraction conditions for polysaccharides from the stems of A. gracilistylus were as follows: material-liquid ratio was 1∶13.2, the extraction temperature was 80 ℃, and the extraction time was 420 s. With the best extraction conditions, the yield of polysaccharides from the stems of A. gracilistylus was 0.78%. The yield by STE was higher than those by reflux extraction and ultrasonic extraction in this research. In addition, the bioassay results showed the polysaccharides had no significant toxicity on RAW 264.7 cells at the dose of 10－20 μg/mL and facilitated the secretion of nitric oxide (NO) in the cells supernatant at the dose of 10－40 μg/mL in vitro. Conclusion These results establish a steady and convenient extraction technology for polysaccharides from the stems of A. gracilistylus using STE method, indicating the polysaccharides have immunological activity to some extent, which provides a theoretical basis for the reasonable development of A. gracilistylus resources.

湖南省自然科學(xué)基金重點(diǎn)項(xiàng)目（11JJ2042）；湖南中醫(yī)藥大學(xué)藥物分析學(xué)“十二五”校級(jí)重點(diǎn)學(xué)科建設(shè)項(xiàng)目