目的 為了明確內(nèi)源多胺是否介導(dǎo)真菌誘導(dǎo)子誘導(dǎo)白樺三萜的合成。方法 將40 μg/mL真菌誘導(dǎo)子、1 mmol/L腐胺（Put）和2 mmol/L多胺合成抑制劑D-精氨酸（D-Arg）添加到培養(yǎng)8 d的白樺懸浮培養(yǎng)體系中，利用高效液相色譜和比色法分析多胺量和三萜量。采用藥理學(xué)和恢復(fù)實驗分析多胺在真菌誘導(dǎo)子誘導(dǎo)白樺三萜合成中的作用。結(jié)果 真菌誘導(dǎo)子或Put處理后，白樺懸浮細(xì)胞中的多胺量、三萜量和產(chǎn)量均呈增加趨勢，其中處理24 h時，三萜量達(dá)最大值，分別增加了68.54%和30.34%。真菌誘導(dǎo)子和Put共同處理雖提高了三萜量，但其產(chǎn)量卻低于真菌誘導(dǎo)子單獨處理。真菌誘導(dǎo)子和D-Arg共同處理后三萜量低于真菌誘導(dǎo)子單獨處理，處理24 h時降低程度最高，為40.57%?；謴?fù)實驗發(fā)現(xiàn)，隨著恢復(fù)時間的延長，真菌誘導(dǎo)子、Put以及真菌誘導(dǎo)子與D-Arg對白樺三萜合成的影響效應(yīng)逐漸減弱，恢復(fù)到對照水平。結(jié)論 多胺介導(dǎo)了真菌誘導(dǎo)子促進(jìn)白樺懸浮細(xì)胞中三萜的合成。

Objective To clarify whether polyamine mediated triterpenoid synthesis in birch (Betula platyphylla) suspension cells induced by fungal elicitor. Methods Fungal elicitor (40 μg/mL), putrescine (Put, 1 mM), and D-arginine (D-Arg, 2 mM) were added to the eight-day-old suspension cell culture, the changes of triterpenoid content and free polyamines content (free Put, Spm and Spd) were analyzed by chemical colorimetry and HPLC. The effect of polyamine on the triterpenoid synthesis in birch (Betula platyphylla) suspension cells induced by fungal elicitor was analyzed by pharmacology and restoration experiment. Results After the treatment of fungal elicitor or Put, the contents and yields of free polyamines and triterpenoid were increased. Among of them, the triterpenoid content was the highest after 24 h treatment, the increasing rates were 68.54% and 30.34%, respectively. The triterpenoid content was increased by the cotreatment of fungal elicitor and Put, but the increasing degree of yield was lower than that by the treatment of fungal elicitor alone. Comparing with the fungal elicitor alone, the cotreatment of fungal elicitor and D-Arg decreased the triterpenoid content by 40.57% in the highest degree of decreasing after 24 h treatment. In restoration experiment, with the treatment time prolonging, the effect of fungal elicitor, Put, or cotreatment of fungal elicitor and D-Arg on triterpenoid synthesis was decreasing to the level of the control finally. Conclusion From the above results, we conclude that polyamine could mediate the synthesis of triterpenoid in cells of B. platyphylla induced by fungal elicitor.

中央高效基本科研業(yè)務(wù)專項資金項目（DL13EA08-03）；國家自然基金項目（31100445和31070531）；黑龍江省博士后科研啟動金（415288）